Integrative omics study on bone metastatic tumors using clinical samples for clinical applications

利用临床样本进行骨转移肿瘤的综合组学研究用于临床应用

• 批准号: 22501057
• 负责人: KONDO Tadashi
• 金额: $ 2.83万
• 依托单位: National Cancer Center Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22501057/
• 关键词: 化学療法; 転移性骨腫瘍; 創薬標的; プロテオーム解析; 網羅的解析

To reveal the molecular backgrounds of metastatic bone tumor, we created the expression profiles of primary tumor tissues and bone metastatic tissues, which were obtained and storaged at the time of surgery. Lung cancers, breast cancers, hepatocellular carcinomas, colorectal cancers, pancreatic cancers, gastric cancers, renal cell carcinomas, and parotid glad tumors were included this study. Protein expression profiles were created by two-dimensional difference gel electrophoresis, and PROTOMAP. Global microRNA expression was examined by the microarray. We identified genes whose expression levels were aberrantly regulated in the bone-metastatic tumors compared with the primary tumors. The functional significances of the identified genes were verified by the in vitro experiments.

为了揭示转移性骨肿瘤的分子背景，我们建立了原发性肿瘤组织和骨转移组织的表达谱，这些组织是在手术时获得和储存的。本研究包括肺癌、乳腺癌、肝细胞癌、结直肠癌、胰腺癌、胃癌、肾细胞癌和腮腺恶性肿瘤。通过二维差异凝胶电泳和PROTOMAP建立蛋白质表达谱。通过微阵列检查总体microRNA表达。我们鉴定了与原发肿瘤相比，骨转移肿瘤中表达水平异常调节的基因。通过体外实验验证了所鉴定基因的功能意义。

项目成果

Peroxiredoxin 2 as a chemotherapy responsiveness biomarker candidate in osteosarcoma revealed by proteomics

• DOI: 10.1002/prca.200900172
• 发表时间: 2010-05-01
• 期刊: PROTEOMICS CLINICAL APPLICATIONS
• 影响因子: 2
• 作者: Kikuta, Kazutaka;Tochigi, Naobumi;Kondo, Tadashi
• 通讯作者: Kondo, Tadashi

Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis

• DOI: 10.1038/labinvest.2012.98
• 发表时间: 2012-09-01
• 期刊: LABORATORY INVESTIGATION
• 影响因子: 5
• 作者: Ito, Akihiko;Mimae, Takahiro;Kondo, Tadashi
• 通讯作者: Kondo, Tadashi

Proteomics application for sarcoma

蛋白质组学在肉瘤中的应用

• 发表时间: 2012
• 作者: Kikuta K;et al;Kondo T;近藤格;Kondo T
• 通讯作者: Kondo T

Proteomic approach for biomarker discovery towards personalized medicine

用于个性化医疗生物标志物发现的蛋白质组学方法

• 发表时间: 2012
• 作者: Kondo T;Kubota D;Ichikawa H;Sugihara Y;Sekizaki Y;Murakami Y;Huang C;Fan J;Kawai A
• 通讯作者: Kawai A

プロテオーム解析による個別化医療のためのバイオマーカー開発

通过蛋白质组分析开发个性化医疗的生物标志物

• 作者: 鈴木昭久;宮本 強;鹿島大靖;塩沢丹里;Hamatani T;落谷孝広;塩沢丹里;近藤格
• 通讯作者: 近藤格