Spatiotemporal alteration of chromatin structure at promoter regions

启动子区域染色质结构的时空变化

• 批准号: 22570006
• 负责人: ISHIHARA Naoe
• 金额: $ 2.83万
• 依托单位: Fujita Health University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570006/
• 关键词: アロマターゼ; クロマチン; ヒストン; プロモーター; 転写調節

We analyzed chromatin structure responsible to the alternativepromoter usage of the aromatase gene (CYP19A1), which is transcribed from three majorpromoters. Our novel method using sedimentation velocity centrifugation revealed thatworking promoters were exclusively composed of open chromatin. In contrast, therecruitment of trimethylation of histone H3 lysine 27, which is known as a negative markfor transcription, was observed around unused promoters, suggesting that polycombproteins repressed the promoters selectively.

我们分析了负责芳香化酶基因（CYP19A1）的交替启动子使用的染色质结构，该基因由三个主要启动子转录。我们采用沉降速度离心的新方法揭示了工作启动子完全由开放的染色质组成。相反，在未使用的启动子周围观察到组蛋白H3赖氨酸27的三甲基化的募集，这被认为是转录的负标记，这表明多梳蛋白选择性地抑制启动子。

项目成果

A new fractionation assay, based on the size of formaldehyde-crosslinked, mildly sheared chromatin, delineates the chromatin structure at promoter regions.

• DOI: 10.1093/nar/gkq203
• 发表时间: 2010-06
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Ishihara S;Varma R;Schwartz RH
• 通讯作者: Schwartz RH

CYP19遺伝子のプロモーター選択機構:密なクロマチン構造でプロモーターを選択的に抑制する

CYP19基因的启动子选择机制：致密染色质结构对启动子的选择性抑制

• 发表时间: 2012
• 作者: Oguro R;Kamide K;Katsuya T;Akasaka H;Sugimoto K;Congrains A;Arai Y ;Hirose N;Saitoh S;Ohishi M;Miura T;Rakugi H;石原悟,琴村直恵,原田信広
• 通讯作者: 石原悟,琴村直恵,原田信広

Two-step binding of transcription factors causes sequential chromatin structural changes at the activated IL-2 promoter.

• DOI: 10.4049/jimmunol.1003173
• 发表时间: 2011-09-15
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Ishihara S;Schwartz RH
• 通讯作者: Schwartz RH

The role of IL-15 in activating STAT5 and fine-tuning IL-17A production in CD4 T lymphocytes.

• DOI: 10.4049/jimmunol.1201476
• 发表时间: 2012-11-01
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Pandiyan P;Yang XP;Saravanamuthu SS;Zheng L;Ishihara S;O'Shea JJ;Lenardo MJ
• 通讯作者: Lenardo MJ

Complex cell cycle abnormalities caused by human T-lymphotropic virus type 1 Tax.

由人类 T 淋巴细胞病毒 1 型 Tax 引起的复杂细胞周期异常。

• DOI: 10.1128/jvi.00086-10
• 发表时间: 2011
• 期刊: Journal of virology
• 影响因子: 5.4
• 作者: Yang,Liangpeng;Kotomura,Naoe;Ho,Yik-Khuan;Zhi,Huijun;Bixler,Sandra;Schell,MichaelJ;Giam,Chou-Zen
• 通讯作者: Giam,Chou-Zen