Expression of novel carbohydrate-specific single chain antibodies in E. coli and analyses of carbohydrate-binding activities of purified antibodies

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Aberration of carbohydrates on cell surface is often associated with pathogenic changes in diseases. Towards developing anti-cancer therapeutics, we had obtained genes for anti-carbohydrate single chain antibodies (scFvs) against carbohydrate moieties expressed specifically in cancer cells using phage-display methods. In this study, expression of scFv genes in E. coli and insect cells was successfully carried out to produce antibody proteins, whose carbohydrate binding activities were confirmed.