Establishment of embryonic stem cell culture for high efficient generation of transgenic chick

高效产生转基因鸡的胚胎干细胞培养物的建立

• 批准号: 22570177
• 负责人: MAK Siu Shan
• 金额: $ 3万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570177/
• 关键词: 組換え; 胚性幹細胞

In this project, we set out to establish pluripotent cell culture in the zebra finch species. We found that finch eggs were laid at an earlier stage than chick. There were significant differences between the unincubated eggs of these two species in terms of cell identity and cell behaviour in vitro. As a consequence dissociated finch cells, but not chick cells, produced cells that expressed various stem cell markers under a defined culture condition. We reported for the first time, a detailed molecular analysis of post-morula avian embryos and a methodology for culturing such early embryos and a feeder-free serum-free culture condition that could obtain stem cell-like cells.

在这个项目中，我们着手在斑马雀物种中建立多能细胞培养。我们发现，雀鸟蛋比雏鸟更早下蛋。这两种未孵化的卵在细胞特性和体外细胞行为方面存在显著差异。结果，分离的雀类细胞，而不是鸡细胞，在特定的培养条件下产生了表达各种干细胞标记的细胞。我们首次报道了对桑椹胚后的禽类胚胎进行详细的分子分析，以及培养这种早期胚胎的方法，以及一种可以获得干细胞样细胞的无饲养层无血清培养条件。

项目成果

Towards establishing the zebra finch as a chimeric avian model

建立斑胸草雀作为嵌合鸟类模型

• 发表时间: 2013
• 作者: 田上英明;加藤麻希;伊縫美妃;田上英明;Siu Shan Mak
• 通讯作者: Siu Shan Mak

A study on early pre-gastrula stages of the zebra finch blastoderm and its pluripotent potential

斑胸草雀胚层早期前原肠胚阶段及其多能潜能的研究

• 发表时间: 2012
• 作者: 田上英明;加藤麻希;伊縫美妃;田上英明;Siu Shan Mak;Siu Shan Mak
• 通讯作者: Siu Shan Mak

Characterising the zebra finch blastodermal cells

斑胸草雀胚层细胞的特征

• 发表时间: 2012
• 作者: 田上英明;加藤麻希;伊縫美妃;田上英明;Siu Shan Mak;Siu Shan Mak;Siu Shan Mak
• 通讯作者: Siu Shan Mak

Establishing the zebra finch as a model system for developmental studies

建立斑胸草雀作为发育研究的模型系统

• 发表时间: 2012
• 作者: 田上英明;加藤麻希;伊縫美妃;田上英明;Siu Shan Mak;Siu Shan Mak;Siu Shan Mak;Siu Shan Mak
• 通讯作者: Siu Shan Mak