Establishment of embryonic stem cell culture for high efficient generation of transgenic chick

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In this project, we set out to establish pluripotent cell culture in the zebra finch species. We found that finch eggs were laid at an earlier stage than chick. There were significant differences between the unincubated eggs of these two species in terms of cell identity and cell behaviour in vitro. As a consequence dissociated finch cells, but not chick cells, produced cells that expressed various stem cell markers under a defined culture condition. We reported for the first time, a detailed molecular analysis of post-morula avian embryos and a methodology for culturing such early embryos and a feeder-free serum-free culture condition that could obtain stem cell-like cells.