Analysis of the ubiquitin-ligase complex in the endoplasmic reticulum membrane that regulates endoplasmic reticulum-associated degradation in mammals

分析内质网膜中调节哺乳动物内质网相关降解的泛素连接酶复合物

• 批准号: 22570183
• 负责人: HOSOKAWA Nobuko
• 金额: $ 2.91万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570183/
• 关键词: 小胞体; 小胞体関連分解; ユビキチンリガーゼ; タンパク質品質管理; HRD1-SEL1L複合体; レクチン; タンパク質; ユビキチンプロテアソーム系; 糖鎖; タンパク質分解; 分子細胞生物学; HRD1-SEL1L

Endoplasmic reticulum (ER) is an organelle in which v a r i o u s kinds of proteins are synthesized. Newly synthesized proteins that failed to obtain their native conformations are retrotranslocated out of the ER, and degraded by the cytoplasmic proteasome after the addition of u b i q u i t i n s b y t h e u b i q u i t i n l i g a s e c o m p l e x t h a t r e s i d e s in the ER membrane. In the present study, we have analyzed the stability of each component of the complex and found that the dynamic change of the component o f t h e c o m p l e x r e g u l a t e s the degradation of misfolded substrates.

内质网（ER）是一种细胞器，其中合成了多种蛋白质。 Newly synthesized proteins that failed to obtain their native conformations are retrotranslocated out of the ER, and degraded by the cytoplasmic proteasome after the addition of u b i q u i t i n s b y t h e u b i q u i t i n l i g a s e c o m p l e x t h a t r e s i d e s in the ER membrane.在本研究中，我们已经分析了复合物的每个组件的稳定性，并发现成分的动态变化与错误折叠底物的降解降解。

项目成果

Functional analysis of a mammalian lectin XTP3-B in the endoplasmic reticulum quality control.

哺乳动物凝集素 XTP3-B 在内质网质量控制中的功能分析。

• 发表时间: 2010
• 作者: Fujimori T;Kamiya Y;Kato K;Nagata K;Hosokawa N
• 通讯作者: Hosokawa N

Formation of HRD1-SEL1L ubiquitin ligase complex that regulates the mammalian ERAD.

形成调节哺乳动物 ERAD 的 HRD1-SEL1L 泛素连接酶复合物。

• 发表时间: 2010
• 作者: Hosokawa N;Iida Y;Okawa K;Nagata K.
• 通讯作者: Nagata K.

Roles of N-glycans in the regulation of mammalian glycoprotein quality control.

N-聚糖在哺乳动物糖蛋白质量控制中的作用。

• 发表时间: 2011
• 作者: Hosokawa N;Kamiya Y;Kato K.;Hosokawa N;Hosokawa N;Hosokawa N
• 通讯作者: Hosokawa N

The acidic environment of the Golgi is critical for glycosylation and transport

高尔基体的酸性环境对于糖基化和运输至关重要

• DOI: 10.1016/s0076-6879
• 发表时间: 2010
• 期刊: Methods Enzymol
• 作者: Maeda;Y. and T. Kinoshita
• 通讯作者: Y. and T. Kinoshita

EDEM1 accelerates the trimming of α1,2-linked mannose on the C branch of N-glycans

• DOI: 10.1093/glycob/cwq001
• 发表时间: 2010-05-01
• 期刊: GLYCOBIOLOGY
• 影响因子: 4.3
• 作者: Hosokawa, Nobuko;Tremblay, Linda O.;Herscovics, Annette
• 通讯作者: Herscovics, Annette