The molecular analysis of the development of cerebellum by using zebrafish mutants

利用斑马鱼突变体对小脑发育进行分子分析

• 批准号: 22570201
• 负责人: SHIMIZU Takashi
• 金额: $ 3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570201/
• 关键词: 細胞分化; 神経分化; 小脳; 顆粒細胞; 神経発生; 神経回路; ゼブラフィッシュ

To understand the genes that control the development of cerebellar neurons, we investigated the zebrafish mutants gazami(gaz) and shiomaneki(sio).The gaz mutant larvae showed a strong reduction of granule cells. By the positional cloning, we mapped the gaz mutation to the gene encoding a nuclear protein, cranio facial development protein1(cfdp1). In the anterior neuroectoderm, the number of apoptotic cells was increased in the mutant larvae. We also found that proliferating cells were increased in mutants at 4 dpf. Our findings suggest that Cfdp1 function in the differentiation from the neural progenitors and/or proliferation of neural progenitor cells.The sio mutants displayed mistargeted axon of granule cells. The sio mutation mapped to the gene encodes type IV collagen a6 whish serve as a component of basement membrane.The sio larvae showed the disruption of basement membrane structure. These results suggest that the interaction between neuron and basement membrane is important for the axogenesis of cerebellar granule cells.

为了了解控制小脑神经元发育的基因，我们研究了斑马鱼突变体gazami(Gaz)和shiomane ki(Sio)。通过定位克隆，我们将GAZ突变定位于核蛋白cfdp1(Cfdp1)。在前神经外胚层，突变型幼虫的凋亡细胞数增加。我们还发现，在4dpf时，突变体的增殖细胞增加。我们的发现提示Cfdp1在神经前体细胞的分化和/或神经前体细胞的增殖中起作用。SIO突变定位于编码IV型胶原a6型的基因，是基底膜的组成部分，SIO幼虫表现为基底膜结构破坏。这些结果表明，神经元与基底膜的相互作用对小脑颗粒细胞的轴突发生具有重要意义。

项目成果

名古屋大学生物機能開発利用研究センター動物器官機能研究分野

名古屋大学生物功能开发利用研究中心动物器官功能研究部

Genetic control of development of cerebellar neurons and neural circuits in zebrafish

斑马鱼小脑神经元和神经回路发育的遗传控制

• 发表时间: 2011
• 作者: Shimizu;T.;Kani;S.;Bae;Y.-K.,Tanabe;K.;Kusuda;R.;and Hibi;M.
• 通讯作者: M.

Genetic control for development of cerebellar neurons and neural circuits in zebrafish

斑马鱼小脑神经元和神经回路发育的遗传控制

• 发表时间: 2011
• 作者: Takeuchi;M.;Shimizu;T.;Kani;S.;Bae;Y.-K.;Tanabe;K.;Kusuda;R.*;Asakawa;K.;Kawakami;K.;and Hibi;M.
• 通讯作者: M.

Proneural gene-linked neurogenesis in zebrafish cerebellum

• DOI: 10.1016/j.ydbio.2010.03.024
• 发表时间: 2010-07-01
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Kani, Shuichi;Bae, Young-Ki;Hibi, Masahiko
• 通讯作者: Hibi, Masahiko

Zinc-finger genes Fezf1 and Fezf2 control neuronal differentiation by repressing Hes5 expression in forebrain

锌指基因 Fezf1 和 Fezf2 通过抑制前脑 Hes5 表达来控制神经元分化

• 发表时间: 2010
• 期刊: Development
• 影响因子: 4.6
• 作者: Shimizu;T.;Nakazawa;M.;Kani;S.;Bae;Y.-K.;Shimizu;T.;Kageyama;R.;Hibi;M.
• 通讯作者: M.