The molecular analysis of the development of cerebellum by using zebrafish mutants

利用斑马鱼突变体对小脑发育进行分子分析

• 批准号: 22570201
• 负责人: SHIMIZU Takashi
• 金额: $ 3万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22570201/
• 关键词: 細胞分化; 神経分化; 小脳; 顆粒細胞; 神経発生; 神経回路; ゼブラフィッシュ

To understand the genes that control the development of cerebellar neurons, we investigated the zebrafish mutants gazami(gaz) and shiomaneki(sio).The gaz mutant larvae showed a strong reduction of granule cells. By the positional cloning, we mapped the gaz mutation to the gene encoding a nuclear protein, cranio facial development protein1(cfdp1). In the anterior neuroectoderm, the number of apoptotic cells was increased in the mutant larvae. We also found that proliferating cells were increased in mutants at 4 dpf. Our findings suggest that Cfdp1 function in the differentiation from the neural progenitors and/or proliferation of neural progenitor cells.The sio mutants displayed mistargeted axon of granule cells. The sio mutation mapped to the gene encodes type IV collagen a6 whish serve as a component of basement membrane.The sio larvae showed the disruption of basement membrane structure. These results suggest that the interaction between neuron and basement membrane is important for the axogenesis of cerebellar granule cells.

为了了解控制小脑神经元发展的基因，我们研究了斑马鱼突变体Gazami（GAZ）和Shiomaneki（SIO）。GAZ突变幼虫显示出颗粒细胞的强烈减少。通过位置克隆，我们将GAZ突变映射到编码核蛋白Cranio面部发育蛋白1（CFDP1）的基因。在神经外胚层中，突变幼虫中凋亡细胞的数量增加。我们还发现，在4 dpf的突变体中增加了增殖细胞。我们的发现表明，CFDP1在与神经祖细胞和/或神经祖细胞的增殖的分化中起作用。SIO突变体显示了颗粒细胞的瞬间轴突。映射到该基因的SIO突变编码IV型胶原蛋白A6 Whish用作基底膜的组成部分。SIO幼虫显示了基底膜结构的破坏。这些结果表明，神经元与基底膜之间的相互作用对于小脑颗粒细胞的轴发生很重要。

项目成果

Genetic control of development of cerebellar neurons and neural circuits in zebrafish

斑马鱼小脑神经元和神经回路发育的遗传控制

• 发表时间: 2011
• 作者: Shimizu;T.;Kani;S.;Bae;Y.-K.,Tanabe;K.;Kusuda;R.;and Hibi;M.
• 通讯作者: M.

Proneural gene-linked neurogenesis in zebrafish cerebellum

• DOI: 10.1016/j.ydbio.2010.03.024
• 发表时间: 2010-07-01
• 期刊: DEVELOPMENTAL BIOLOGY
• 影响因子: 2.7
• 作者: Kani, Shuichi;Bae, Young-Ki;Hibi, Masahiko
• 通讯作者: Hibi, Masahiko

Genetic control for development of cerebellar neurons and neural circuits in zebrafish

斑马鱼小脑神经元和神经回路发育的遗传控制

• 发表时间: 2011
• 作者: Takeuchi;M.;Shimizu;T.;Kani;S.;Bae;Y.-K.;Tanabe;K.;Kusuda;R.*;Asakawa;K.;Kawakami;K.;and Hibi;M.
• 通讯作者: M.

IV型コラーゲンはゼブラフィッシュにおける小脳顆粒細胞の軸索伸長に必要である

斑马鱼小脑颗粒细胞轴突生长需要 IV 型胶原蛋白

• 发表时间: 2012
• 作者: 佐藤 智典;高橋 良尚;水野 真盛;竹内未紀
• 通讯作者: 竹内未紀

A nuclear protein Cfdp1 is involved in differentiation of granule cells in cerebellum

核蛋白 Cfdp1 参与小脑颗粒细胞的分化

• 发表时间: 2013
• 作者: Nejigane S;et al.;Takuya Minokawa;清水貴史
• 通讯作者: 清水貴史