Conversion of hydrolases into transglycosidases
Conversion of hydrolases into transglycosidases
批准号:
22580099
负责人:
MORI Haruhide
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012
中文摘要
碳水化合物水解酶可以产生多种低聚糖。在这项工作中,通过转糖苷酶和水解酶的比较,确定了负责高转糖基化活性的氨基酸残基。结果表明,有一种转糖苷酶的催化结构域类似于异构体转化水解酶,而后者不具有转糖苷酶活性。
英文摘要
A variety of oligosaccharides can be produced by carbohydrate hydrolases. In this work, amino acid residues responsible for high transglycosylation activities were identified through the comparison of transglycosidases with hydrolases. It was clarified that one transglycosidase had a catalytic domain similar to anomer-inverting hydrolases, which possess no transglycosidase activity.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Isomaltooligosaccharide 6-α-glucosyltransferaseのサブサイト+ 1変異酵素の機能解析
低聚异麦芽糖6-α-葡萄糖基转移酶亚位点+1突变酶的功能分析
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[西村崇志, 鐘ケ江倫世, 本同宏成, 奥山正幸, 森春英, 木村淳夫,]
通讯作者:
木村淳夫,
Domain organization and catalytic amino acids of dextran dextrinase: structural similarity to inverting glucodextanase (or glucoamylase) in catalytic domain".
葡聚糖糊精酶的结构域组织和催化氨基酸:催化结构域中与转化葡糖葡聚糖酶(或葡糖淀粉酶)的结构相似性”。
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Sadahiro, J., Mori, H.]
通讯作者:
H.
Identification of a key residue for regioselectivity of α-glucosidase from Schwanniomyces occidentalis
西许旺酵母 α-葡萄糖苷酶区域选择性关键残基的鉴定
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Kohtaro Kirimura, Yuki Honda, Takasumi Hattori, Kyung-Mo Song, 長谷川大・小田忍・大箸信一, 小野寺威文, Kyung-Mo Song]
通讯作者:
Kyung-Mo Song
Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans
变形链球菌葡聚糖酶的结晶和初步晶体学分析
DOI:
10.1107/s1744309111038425
发表时间:
2011
期刊:
Acta Crystallogr F
影响因子:
0.9
作者:
[Suzuki N, Kim YM, Fujimoto Z, Momma M, Kang HK, Funane K, Okuyama M, Mori H,Kimura A]
通讯作者:
Mori H,Kimura A
DOI:
10.1007/s00253-011-3201-y
发表时间:
2011-07-01
期刊:
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
影响因子:
5
作者:
[Kim, Young-Min, Shimizu, Ryoko, Kimura, Atsuo]
通讯作者:
Kimura, Atsuo
共 46 条
Diversification of carbohydrate phosphorylases
-
批准号:19K22263
-
项目类别:Grant-in-Aid for Challenging Research (Exploratory)
-
资助金额:$3.99万
-
财政年份:2019
-
负责人:MORI Haruhide
-
依托单位:
Utilization of enzymes for production of a variety of carbohydrates
-
批准号:18H02133
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.48万
-
财政年份:2018
-
负责人:MORI Haruhide
-
依托单位:
Molecular Mechanism and Molecular Evolution of Glycosidases
-
批准号:12460035
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$9.22万
-
财政年份:2000
-
负责人:MORI Haruhide
-
依托单位:
Research Project on Teaching and Hosting Foreigh Students
-
批准号:02451036
-
项目类别:Grant-in-Aid for General Scientific Research (B)
-
资助金额:$2.05万
-
财政年份:1990
-
负责人:MORI Haruhide
-
依托单位:
海外基金