Comprehensive analysis of the biosynthetic machinery and their regulatory system for secondary metabolites in Streptomyces

对罗氏链霉菌7434AN4产生兰卡西丁和兰卡霉素的生物合成机制及其调控系统进行了全面的分析。编码II型硫代酯酶的LkmE的干扰物积累了15-Nor-LM衍生物，这表明LkmE是导致LM生物合成的异常起始单位的水解物。我们阐明了诱导LC和LM产生的信号分子SRBS的结构。此外，对调控基因的广泛分析揭示了两个SARP基因对LM生物合成的正调控作用。

We carried out comprehensive analysis of the biosynthetic machinery and their regulatory system for lankacidin and lankamycin production in Streptomyces rochei 7434AN4. The disruptant of lkmE encoding type-II thioesterase accumulated 15-nor-LM derivatives, suggesting LkmE is responsible for hydrolysis of aberrant starter units for LM biosynthesis. We elucidated the structure of the signaling molecules SRBs that induce LC and LM production. Furthermore, the extensive analysis of regulatory genes revealed the positive regulation of LM biosynthesis by two SARP genes.