Improvement of somatic cell nuclear transfer techniques in miniature pigs using a noninvasive evaluation system for reprogramming status

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It was indicated that treatment with some histone deacetylase inhibitors, such as valproic acid, suberoyl bishydroxamic acid and scriptaid, is beneficial for the efficient production of miniature pig somatic cell nuclear transfer embryos using a noninvasive evaluation system for the reprogramming status of donor nuclei on the basis of Oct-3/4 expression. In addition, an alive transgenic miniature pig expressing human apolipoprotein(a), a risk factor for the development of atherosclerosis, was successfully obtained using treatment with scriptaid.