Transcriptomic profiling of skeletal muscle microRNA involved in muscle fiber type specification

参与肌纤维类型规范的骨骼肌 microRNA 的转录组分析

• 批准号: 22580326
• 负责人: MUROYA Susumu
• 金额: $ 2.91万
• 依托单位: National Agriculture and Food Research Organization
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580326/
• 关键词: ウシ; 筋線維型; 骨格筋; マイクロRNA; 次世代シーケンサー

To elucidate roles of miRNA in bovine muscle type specification and maintenance, we sought to determine differentially expressed miRNA between semitendinosus (STD) and masseter (MS) muscles from 3 Japanese black cattle by massively parallel sequencing. Differential gene expression of myosin heavy chain (MyHC) isoforms confirmed that STD and MS were MyHC-2x- and MyHC-1-abundant muscles, respectively. In total, 192 known miRNA and 20 potential new bovine miRNA were obtained from the sequencing. The differentially expressed miRNA with more than 2-fold difference in each muscle were identified. In particular, miR-196a and miR-885 were exclusively expressed in STD muscle, which was validated by quantitative reverse transcription-PCR (P=0.045 and P<0.001, respectively), whereas a slow type-directing miR-208b was highly expressed in MS compared with STD (false discovery rate<0.05). In addition, 16 potential novel miRNA were mapped and confirmed for their precursor structures by computational analyses.

为了阐明miRNA在牛肌肉类型特化和维持中的作用，我们试图通过大规模平行测序来确定3头日本黑牛半腱肌（STD）和咬肌（MS）之间差异表达的miRNA。肌球蛋白重链（MyHC）亚型的差异基因表达证实，STD和MS分别是MyHC-2x-和MyHC-1-丰富的肌肉。共获得192个已知的miRNA和20个潜在的新的牛miRNA。鉴定了在每块肌肉中差异超过2倍的差异表达的miRNA。特别是，miR-196 a和miR-885仅在STD肌肉中表达，这通过定量逆转录-PCR验证（分别为P=0.045和P<0.001），而与STD相比，慢型定向miR-208 b在MS中高度表达（假发现率<0.05）。此外，16个潜在的新的miRNA被映射，并通过计算分析确认其前体结构。

项目成果

Effects of muscle type on beef taste-traits assessed by an electric sensing system

• DOI: 10.1111/j.1740-0929.2010.00773.x
• 发表时间: 2010-01-01
• 期刊: ANIMAL SCIENCE JOURNAL
• 影响因子: 2
• 作者: Chikuni, Koichi;Oe, Mika;Muroya, Susumu
• 通讯作者: Muroya, Susumu

牛肉熟成中のドリップにおけるトロポニンTペプチド含量の経時変化

熟成过程中牛肉滴中肌钙蛋白T肽含量的时程

• 发表时间: 2012
• 作者: 室谷進;大江美香;柴田昌宏;中島郁世;尾嶋孝一;千國幸一
• 通讯作者: 千國幸一

Effects of grass-fed and grain-fed diets on expression of genes for muscle development in Japanese Black steers

草饲和谷物饲粮对日本黑牛肌肉发育基因表达的影响

• 发表时间: 2010
• 作者: 中島郁世;大江美香;尾嶋孝一;室谷進;柴田昌宏;千國幸一;M.Shibata
• 通讯作者: M.Shibata

放牧及び飼料イネWCS給与が黒毛和種去勢牛の肥育成績、筋肉内遺伝子発現に及ぼす影響

放牧和水稻WCS饲喂对日本黑牛育肥性能和肌内基因表达的影响

• 发表时间: 2013
• 作者: 水戸友美;吉岡耕治;鈴木千恵;山下祥子;星宏良;柴田昌宏,松本和典,曳野泰子,室谷進,大江美香,中島郁世,千國幸一,大谷一郎,山本直幸
• 通讯作者: 柴田昌宏,松本和典,曳野泰子,室谷進,大江美香,中島郁世,千國幸一,大谷一郎,山本直幸

Fatty acids modulate adipocyte growth and development in pig : an approach from cell culture study

脂肪酸调节猪脂肪细胞的生长和发育：细胞培养研究的一种方法

• 发表时间: 2011
• 作者: 中島郁世;大江美香;尾嶋孝一;室谷進;柴田昌宏;千國幸一
• 通讯作者: 千國幸一