Generation of transgenic chicken for visualization of germ cells

鸡生殖细胞的可视化对于转基因鸡的产生和了解生殖细胞的分化和增殖具有重要意义。作为第一步，搜索鸡vasa同源物（CVH）启动子序列。我们尝试生产转基因鸡品系，其中生殖系细胞通过CVH启动子驱动的YFP或GFP表达而可视化。而转基因鸡的生殖腺中没有生殖细胞表达荧光信号。

Visualization of chicken germ cells is useful to generate for transgenic chickens and critical to understanding the differentiation and proliferationof germ cells. As a first step, chicken vasa homologue (CVH) promoter sequence was searched. We tried to produce the transgenic chicken line in which the germline cells were visualized by CVH promoter-driven YFP or GFP expression. However, no germ cells in gonads of thetransgenic chickens expressed the fluorescence signal.