Higher serum methionine levels as a predictive factor in patients with irreversible fulminant hepatic failure

较高的血清蛋氨酸水平是不可逆暴发性肝衰竭患者的预测因素

• 批准号: 22591397
• 负责人: SATO Kazushige
• 金额: $ 2.75万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591397/
• 关键词: メチオニン; 急性肝不全; 細胞死; 肝再生; 劇症型; アミノグラム; アルギニン; 肝細胞

Fulminant hepatic failure (FHF) is characterized by acute massive liver necrosis in the absence of chronic liver disease, and also shows an imbalance of the amino acid levels that results from the acute necrosis of hepatocytes. In this study, we investigated the plasma free amino acid profiles in rats with FHF. Acute liver injury was induced by an intragastric administration of 4-hydroxyace-tanilide (APAP) diluted in saline in doses of 7.5 and 3.75 g of APAP per kg b.w. Animals were sacrificed at 1, 6, 12 and 24 hours after treatment. The pre-treatment amino acid profiles of plasma were typical for hepatic failure, with abnormally high levels of phenylalanine, and tyrosine and decreased levels of valine, leucine, and isoleucine. In addition, the levels of many other amino acids such as alanine, lysine, glutamine, methionine, or arginine increased. Among these increased serum amino acids levels, a significant increase of the serum methionine levels was observed in rats with FHF who died, compared to those with FHF who survived after treatments. The markedly increased serum levels of methionine may be associated with the severity of liver damage, which could lead to impaired liver regeneration and multipleorgan failure in patients with FHF.

爆发性肝衰竭（FHF）的特征是在没有慢性肝脏疾病的情况下发生急性大面积肝坏死，并且还表现出肝细胞急性坏死导致的氨基酸水平失衡。在这项研究中，我们研究了血浆游离氨基酸的档案与FHF大鼠。以7.5和3.75 g APAP/kg b.w.的剂量灌胃稀释于生理盐水中的4-羟基乙酰苯胺（APAP），诱导急性肝损伤。在给药后1、6、12和24小时处死动物。治疗前血浆氨基酸谱是肝衰竭的典型特征，苯丙氨酸和酪氨酸水平异常高，缬氨酸、亮氨酸和异亮氨酸水平降低。此外，许多其他氨基酸如丙氨酸、赖氨酸、谷氨酰胺、甲硫氨酸或精氨酸的水平增加。在这些增加的血清氨基酸水平中，与治疗后存活的FHF大鼠相比，在死亡的FHF大鼠中观察到血清甲硫氨酸水平显著增加。血清蛋氨酸水平的显著升高可能与肝损伤的严重程度有关，这可能导致FHF患者的肝再生受损和多器官衰竭。

项目成果

Serum methionine levels as early predictors for survival in patients with fulminant hepatic failure.

血清蛋氨酸水平作为暴发性肝衰竭患者生存的早期预测因子。

• 发表时间: 2010
• 作者: K. Usui;K. Ogawa;E. Kunieda;鈴木 涼子 松井とにか 荻野伊知朗 井上登美夫;Shohei Komatsu;佐藤和重
• 通讯作者: 佐藤和重

Higher serum methionine levels as a predictive factor in patients with irreversible fulminant hepatic

较高的血清蛋氨酸水平是不可逆性暴发性肝病患者的预测因素

• 期刊: failure Transplantation proceedings
• 作者: Sato K;Fukushima D;Doi H;and Satomi S.
• 通讯作者: and Satomi S.

Higher serum methionine levels as a predictive factor in patients with irreversible fulminant hepatic failure.

较高的血清蛋氨酸水平是不可逆暴发性肝衰竭患者的预测因素。

• 发表时间: 2013
• 期刊: Transplantation Proceedings
• 影响因子: 0.9
• 作者: K. Sato;D. Fukushima;H. Doi;S. Satomi
• 通讯作者: S. Satomi