Epigenetics of mesenchymal stem cells considering regenerative therapy for osteoarthritis

从表观遗传学角度分析了人骨髓间充质干细胞的分化潜能。通过对多个基因位点胞嘧啶甲基化状态的分析，推测基质衍生因子1(又称CSCL12)基因上游区域可能是该基因在hMSCs中的沉默元件。尽管胞嘧啶甲基化的改变在体外分化系统中很少见，但全局分析表明，不同组织来源的hMSCs之间存在一致的差异，这可能反映了单个细胞相对较长的表观遗传史。

The differentiating potentials of human mesenchymal stem cells (hMSCs) were analyzed from epigenetic aspect. By analyzing cytosine methylation status of various gene loci, an upstream region of stromal derived factor 1 (also called as CSCL12) gene was hypothesized to function as silencer element of this gene in hMSCs. Although the alteration of cytosine methylation was a rare event in the in vitro differentiation system, global analysis indicated consistent differences could be represented among hMSCs with different tissue origins, which may reflect relatively long term epigenetic history of individual cells.