Functional analysis of the repressive factor of angiogenesis and lymphangiogenesis, CSDA.

血管生成和淋巴管生成抑制因子的功能分析，CSDA。

• 批准号: 22791242
• 负责人: SAITO Yukihiro
• 金额: $ 2.5万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791242/
• 关键词: 実験外科学; 腫瘍血管新生; 腫瘍リンパ管新生; 癌幹細胞; CSDA; アポトーシス; 癌; 遺伝子; 血管新生; リンパ管新生

【Objective】Our objective was to investigate the effect of cold shock domain protein A (CSDA) on carcinogenesis and secretions of angiogenic and lymphangiogenic factors from tumor cells. 【Results】In human skeletal muscle cells (SkMC), CSDA was up-regulated during hypoxia when cells were damaged and apoptosis was induced. CSDA expression could up-regulate the c-fos and VEGF promoter activity on cancer cell lines, CT26, LLC, and AZL5G, but these results were inconsistent with previous reports. We hypothesized that CSDA expresses on cancer non-stem cells and CSDA can control the secretions angiogenic and lymphangiogenic factors from cancer stem cells. We tried to sort the cancer cell line, 4T1, and only 4% cells were recognized as the cancer stem cells.【Conclusion】Given with our in vitro data, we assessed novel hypothesis. Further experiments were required to confirm our concept.

[目的]探讨冷休克蛋白A（CSDA）对肿瘤细胞癌变及血管生成因子和淋巴管生成因子分泌的影响。[结果]在人骨骼肌细胞（SkMC）中，CSDA在缺氧过程中表达上调，细胞受到损伤，诱导细胞凋亡。CSDA表达可上调癌细胞系CT 26、LLC和AZL 5G上的c-fos和VEGF启动子活性，但这些结果与以前的报道不一致。我们假设CSDA在肿瘤非干细胞上表达，并且CSDA可以控制肿瘤干细胞分泌血管生成因子和淋巴管生成因子。我们尝试对癌细胞系4 T1进行分选，只有4%的细胞被识别为癌症干细胞。[结论]结合我们的体外实验数据，我们评估了新的假设。需要进一步的实验来证实我们的概念。

项目成果

Critical Roles of Cold Shock Domain Protein A as an Endogenous Angiogenesis Inhibitor in Skeletal Muscle

冷休克结构域蛋白 A 作为骨骼肌内源性血管生成抑制剂的关键作用

• DOI: 10.1089/ars.2010.3714
• 发表时间: 2011
• 期刊: Antioxidants & Redox Signaling
• 影响因子: 6.6
• 作者: Saito Y;Nakagami H;Azuma N;Hirata S;Sanada F;Taniyama Y;Morishita R;Kaneda Y;Sasajima T
• 通讯作者: Sasajima T

Cold shock domain protein A (CSDA) overexpression inhibits tumor growth and lymph node metastasis in a mouse model of squamous cell carcinoma

冷休克结构域蛋白 A (CSDA) 过表达抑制鳞状细胞癌小鼠模型中的肿瘤生长和淋巴结转移

• DOI: 10.1007/s10585-010-9343-y
• 发表时间: 2010
• 期刊: Clinical and Experimental Metastasis
• 影响因子: 4
• 作者: Matsumoto G;Yajima N;Saito Y;Nakagami H;Omi Y;Lee U;Kaneda Y
• 通讯作者: Kaneda Y

Cold Shock Domain Protein A, Novel Repressor of Tumor-Angiogenesis and Lymphangiogenesis for Cancer Gene Therapy

冷休克结构域蛋白 A，用于癌症基因治疗的肿瘤血管生成和淋巴管生成的新型抑制因子

• 发表时间: 2010
• 作者: Yukihiro Saito;Hironori Nakagami;Nobuyoshi Azuma;Ryuichi Morishita;Tadahiro Sasajima;Yasufumi Kaneda
• 通讯作者: Yasufumi Kaneda

Cold Shock Domain Protein A, as an Endogenous Angiogenesis Inhibitor in Skeletal Muscle

冷休克结构域蛋白 A，作为骨骼肌内源性血管生成抑制剂

• 发表时间: 2010
• 作者: Yukihiro Saito;Hironori Nakagami;Nobuyoshi Azuma;Ryuichi Morishita;Yasufumi Kaneda;Tadahiro Sasajima
• 通讯作者: Tadahiro Sasajima