Development of new efficient cancer-specific artificial viral transduction system

开发新型高效癌症特异性人工病毒转导系统

• 批准号: 22791289
• 负责人: MANABE Tatsuya
• 金额: $ 2.5万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791289/
• 关键词: 膵癌; 人工ウイルス

Through the study of endocytosis pathway in pancreatic cancer, we found that adenovirus transgene expression has been declining which had been higher expression level of integrinβ3 thanβ5. In addition, we can encapsulate gemcitabine and siRNA of integrinβ3 to the protein nanocapsule Mj285(artificial virus) derived from the small heat shock protein produced by originally developed archaea Methanococcusjannaschii. Furthermore we can bind the virus to the antibody MUC1 by amide bond bioconjugation.

通过对胰腺癌细胞内吞途径的研究，发现腺病毒转基因表达量一直在下降，整合素β3的表达水平一直高于β5。此外，我们可以将吉西他滨和整合素β3的siRNA包封到源自最初开发的詹氏甲烷球菌古细菌产生的小热休克蛋白的蛋白纳米胶囊Mj285（人工病毒）上。此外，我们可以通过酰胺键生物缀合将病毒与抗体MUC1结合。

项目成果

High EGFR mRNA expression is a prognostic factor for reduced survival in pancreatic cancer after gemcitabine-based adjuvant chemotherapy

• DOI: 10.3892/ijo.2011.908
• 发表时间: 2011-03-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Fujita, Hayato;Ohuchida, Kenoki;Tanaka, Masao
• 通讯作者: Tanaka, Masao

Gene Expression Levels as Predictive Markers of Outcome in Pancreatic Cancer after Gemcitabine-Based Adjuvant Chemotherapy

• DOI: 10.1593/neo.10458
• 发表时间: 2010-10-01
• 期刊: NEOPLASIA
• 影响因子: 4.8
• 作者: Fujita, Hayato;Ohuchida, Kenoki;Tanaka, Masao
• 通讯作者: Tanaka, Masao

Predicting the chemosensitivity of pancreatic cancer cells by quantifying the expression levels of genes associated with the metabolism of gemcitabine and 5-fluorouracil

• DOI: 10.3892/ijo.2011.1058
• 发表时间: 2011-08-01
• 期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
• 影响因子: 5.2
• 作者: Kurata, Nobuaki;Fujta, Hayato;Tanaka, Masao
• 通讯作者: Tanaka, Masao