Establishment of a novel biomarker of testicular cancer

采用流式细胞术分析小鼠和兔抗DNMT 3A抗体。与正常睾丸组织来源的Hs1细胞无反应。然而，与来自睾丸癌组织的NCCIT细胞有显著的反应，并且该反应被Triton制备物扩增。我们还对人睾丸腺瘤组织进行了DNMT 3A的免疫组织化学分析，并证实人睾丸腺瘤细胞呈阳性染色。

We conducted flow cytometry analysis about DNMT3A antibody of mouse and rabbit. There was no reaction with Hs1 cell arriving from normal testicular tissues. However, there was a significant reaction with NCCIT cell arriving from testicular cancer tissues, and this reaction was amplified by Triton preparation. We also conducted immunohistochemical analysis of DNMT3A for human testicular seminoma tissues, and confirmed that human seminoma cells were positively stained.