Glutamine-glutamate recycle system in the inner ear

内耳中的谷氨酰胺-谷氨酸循环系统

• 批准号: 22791588
• 负责人: OGUCHI Tomohiro
• 金额: $ 2.5万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791588/
• 关键词: 耳科学; グルタミン; 神経伝達物質; 内耳; 難聴; 前庭; グルタミン酸; グルタミン・グルタミン酸サイクル; 遺伝子発現

Glutamate has been implicated in signal transmission between inner hair cells and afferent fibers of the organ of Corti. The inner hair cells are enriched in glutamate and the postsynaptic membranes express AMPA glutamate receptors. However, it is not known whether inner hair cells contain a mechanism for glutamate replenishment. Such a mechanism must be in place to sustain glutamate neurotransmission. Here we provide RT -PCR and immunofluorescence data indicating that system A transporter 1 (SLC38A1), which is associated with neuronal glutamine transport and synthesis of the neurotransmitters GABA and glutamate in CNS, is expressed in inner hair cells.

谷氨酸与Corti器的内毛细胞和传入纤维之间的信号传递有关。内毛细胞富含谷氨酸，突触后膜表达AMPA谷氨酸受体。然而，目前尚不清楚内毛细胞是否含有谷氨酸补充机制。这种机制必须到位，以维持谷氨酸神经传递。在这里，我们提供的RT-PCR和免疫荧光数据表明，系统A转运蛋白1（SLC 38 A1），这是与神经元谷氨酰胺的运输和合成的神经递质GABA和谷氨酸在中枢神经系统，表达在内毛细胞。

项目成果

Vestibular organs express the glutamine transporters

前庭器官表达谷氨酰胺转运蛋白

• 发表时间: 2010
• 作者: 中西啓;水田邦博;大和谷崇;峯田周幸;Oguchi T
• 通讯作者: Oguchi T

Inner hair cells of mice express the glutamine transporter SAT1

• DOI: 10.1016/j.heares.2012.07.005
• 发表时间: 2012-10
• 期刊: Hearing Research
• 影响因子: 2.8
• 作者: T. Oguchi;N. Suzuki;Shigenari Hashimoto;Gauhar Ayub Chaudhry;F. Chaudhry;S. Usami;O. Ottersen

The effect of microgravity on gene expression in the vestibular end-organs. Otoconin 90 was up-regulated by microgravity.

微重力对前庭终末器官基因表达的影响。

• 发表时间: 2012
• 作者: Oguchi T;Nishio S;Suzuki N;Takumi Y;Usami S
• 通讯作者: Usami S

The effect of Microgravity on Gene Expression in the Vestibular Endorgans

微重力对前庭内器官基因表达的影响

• 发表时间: 2011
• 作者: Oguchi T;Suzuki N;Takumi Y;Usami S
• 通讯作者: Usami S