The development of novel decellularization methods : it's application for small diameter vessels

新型脱细胞方法的发展：在小直径血管中的应用

• 批准号: 22791722
• 负责人: SAKAKIBARA Shunsuke
• 金额: $ 2.66万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791722/
• 关键词: 再生医学; 小口径人工血管; 小口径血管; スキャフォールド; 脱細胞化; 再生医療

We developed a novel decellularization method using a hyperosmotic electrolytic solution. The rat abdominal aorta was decellularized and histologically evaluated. These materials, 15 mm in length, were transplanted into the rat abdominal aorta. The hematoxylin and eosin staining and scanning electron microscopic images showed complete decellularization. All transplanted acellular vessels were confirmed to be patent(1 week to 14 months), except in the case of 1 animal, which died 14 months following transplantation. The harvested vessels displayed formation of tunica intima(endothelial cells) and tunica medulla(smooth muscle cells) layers. We also examined the physiological properties of the vessels 3 months after transplantation with a wire myograph system. The transplanted vessels did not react to norepinephrine, but did relax when exposed to sodium nitroprusside. In conclusion, our acellular vessels produced with hyperosmotic electrolytic solution showed extremely high and long-term patency with regeneration of the tunica intima and tunica medulla. In addition, we found that these regenerated acellular vessels possess the physiological properties associated with vascular reflection.

我们开发了一种新的脱细胞方法，使用高渗电解质溶液。对大鼠腹主动脉进行脱细胞并进行组织学评价。这些材料，15毫米长，移植到大鼠腹主动脉。苏木精-伊红染色和扫描电镜图像显示完全脱细胞化。除1只动物在移植后14个月死亡外，所有移植的无细胞血管均被证实通畅（1周至14个月）。收获的血管显示形成图尼卡内膜（内皮细胞）和图尼卡髓质（平滑肌细胞）层。我们还检查了移植后3个月的血管的生理特性与钢丝肌电描记系统。移植的血管对去甲肾上腺素没有反应，但当暴露于硝普钠时会松弛。总之，我们用高渗电解质溶液制备的无细胞血管显示出极高的长期通畅性，图尼卡内膜和图尼卡髓质再生。此外，我们发现这些再生的脱细胞血管具有与血管反射相关的生理特性。

项目成果

A Simplest Method of Flap Monitoring

• DOI: 10.1055/s-0030-1251562
• 发表时间: 2010-09-01
• 期刊: JOURNAL OF RECONSTRUCTIVE MICROSURGERY
• 影响因子: 2.1
• 作者: Sakakibara, Shunsuke;Hashikawa, Kazunobu;Tahara, Shinya
• 通讯作者: Tahara, Shinya

脱細胞化組織を用いた小口径人工血管の開発とその評価

使用脱细胞组织的小直径人造血管的开发和评估

• 发表时间: 2011
• 作者: Sakakibara S;Hashikawa K;Omori M;Terashi H;Tahara S.;榊原俊介
• 通讯作者: 榊原俊介

高張塩溶液脱細胞化法による脱細胞化血管の開発とその評価

高渗盐溶液脱细胞法脱细胞血管的开发与评价

• 发表时间: 2010
• 作者: Sakakibara S;Hashikawa K;Omori M;Terashi H;Tahara S.;榊原俊介;榊原俊介;榊原俊介;榊原俊介;榊原俊介
• 通讯作者: 榊原俊介