The development of novel decellularization methods : it's application for small diameter vessels

我们开发了一种新的使用高渗电解液的脱细胞方法。对大鼠腹主动脉进行脱细胞和组织学评价。这些材料长15 mm，移植到大鼠腹主动脉内。苏木精伊红染色和扫描电子显微镜图像显示细胞完全脱细胞。除1只动物于移植后14个月死亡外，所有移植的无细胞血管均证实开放(1周至14个月)。切取的血管形成内膜(内皮细胞)和髓膜(平滑肌细胞)层。在移植后3个月，我们还用钢丝肌图仪检测了血管的生理特性。移植的血管对去甲肾上腺素没有反应，但在暴露于硝普钠时确实松弛。综上所述，我们用高渗电解液培养的无细胞血管表现出极高的长期通畅性，并可再生内膜和髓膜。此外，我们还发现这些再生的无细胞血管具有与血管反射相关的生理特性。

We developed a novel decellularization method using a hyperosmotic electrolytic solution. The rat abdominal aorta was decellularized and histologically evaluated. These materials, 15 mm in length, were transplanted into the rat abdominal aorta. The hematoxylin and eosin staining and scanning electron microscopic images showed complete decellularization. All transplanted acellular vessels were confirmed to be patent(1 week to 14 months), except in the case of 1 animal, which died 14 months following transplantation. The harvested vessels displayed formation of tunica intima(endothelial cells) and tunica medulla(smooth muscle cells) layers. We also examined the physiological properties of the vessels 3 months after transplantation with a wire myograph system. The transplanted vessels did not react to norepinephrine, but did relax when exposed to sodium nitroprusside. In conclusion, our acellular vessels produced with hyperosmotic electrolytic solution showed extremely high and long-term patency with regeneration of the tunica intima and tunica medulla. In addition, we found that these regenerated acellular vessels possess the physiological properties associated with vascular reflection.