Elucidation of recruitment and recycling of hemidesmosome structural proteins

我们发现，当表皮角质形成细胞运动时，沿运动方向组装的半桥体结构蛋白不会从近端滑动。我们还发现，这些都不是新合成的蛋白质。换句话说，当细胞移动时，在前沿新组装的β4-整合素包含通过内吞作用新循环的蛋白质。如果我们考虑循环所需的时间，很可能是短环内吞作用，这表明需要进一步研究与Rab4等蛋白质的关系。

We found that when epidermal keratinocytes move, the hemidesmosome structural proteins assembled in the direction of movement do not slide from the proximal portion. We also found that these are not newly synthesized proteins. In other words, when cells moveβ4-integrin newly assembled in the leading edge comprises proteins newly recycled through endocytosis. If we consider the time taken to recycle, it is likely that this represents short loop endocytosis, indicating the need for further studies examining the relationship with proteins such as Rab4.