Dentin regeneration by Thy-1-positive dental pulp cells

Thy-1 阳性牙髓细胞的牙本质再生

• 批准号: 22791782
• 负责人: HOSOYA Akihiro
• 金额: $ 2.5万
• 依托单位: Matsumoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791782/
• 关键词: 移植・再生医療; 再生医学; 象牙質; 発生・分化

The majority of cells in the subodontoblastic layer express Thy-1.Therefore, to clarify the characteristics of cells in the subodontoblastic layer, the differentiation ability of Thy-1-positive cells into mineralizing cells was analyzed in vitro and in vivo. Following stimulation with BMP-2, Thy-1-positive dental pulp cells showed accelerated induction of ALP activity. Additionally, subcutaneous implantation of these positive cells induced numerous mineralized matrix formations. These results collectively suggest that Thy-1-positive dental pulp cells localized in the subodontoblastic layer have the ability to differentiate into hard tissue-forming cells.

亚邻孕细胞层中的大多数细胞表达了THY-1。因此，为了阐明亚邻胞细胞层中细胞的特征，在体外和体内分析了THY-1阳性细胞为矿化细胞的分化能力。用BMP-2刺激后，THY-1阳性牙髓细胞表现出ALP活性的加速诱导。另外，这些阳性细胞的皮下植入诱导了许多矿化基质形成。这些结果共同表明，在亚邻育层中定位的THY-1阳性牙髓细胞具有分化为硬组织形成细胞的能力。

项目成果

Thy-1-positive cells in the subodontoblastic layer possess high potential to differentiate into hard tissue-forming cells

• DOI: 10.1007/s00418-012-0928-1
• 发表时间: 2012-06-01
• 期刊: HISTOCHEMISTRY AND CELL BIOLOGY
• 影响因子: 2.3
• 作者: Hosoya, Akihiro;Hiraga, Toru;Nakamura, Hiroaki
• 通讯作者: Nakamura, Hiroaki