Development of value-added chemicals from light using light-driven vesicles and cell factory.

我们从大肠杆菌细胞的内外膜制备了ATP光合囊泡，该囊泡表达delta-视紫红质（一种新型的光驱动H+转运体）和tof1 -ATP合成酶（一种耐热的ATP合成酶）。这些囊泡表现出光依赖性ATP合成。此外，将ATP光合囊泡与ATP水解己糖激酶偶联使光依赖性葡萄糖消耗成为可能。三磷酸腺苷光合囊泡表明了它们在各种三磷酸腺苷水解生物生产的光驱动三磷酸腺苷预生成生物过程中的应用潜力。

We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H+ transporter) and TFoF1-ATP synthase (a thermostable ATP synthase). These vesicles showed lightdependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATPregenerating bioprocess for various ATP-hydrolyzing bioproductions.