Elucidating the molecular mechanisms of the centromere loading of CENP-A at G2 phase

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In fission yeast, it is thought that the incorporation of CENP-A at G2 phase may act as a rescue of the cell lethality. To identify factors that involved in the G2-loading pathway , we screened for multicopy suppressors of G2-loading deficient cells.Five candidate genes are isolated, and one of them has RNA binding motifs. A double mutant lacking both this RNA binding motif and ams2 genes exhibits synthetic lethality. In this study suggest that the factor may be important in centromere deposition of CENP-A at G2 phase.