Clinical Trial of Spinal Cord Regeneration Therapy by Injection of Olfactory Ensheathing Cells(OECs) in Dogs with Spinal Cord Injury

注射嗅鞘细胞（OEC）对脊髓损伤犬进行脊髓再生治疗的临床试验

• 批准号: 22780287
• 负责人: ITO Daisuke
• 金额: $ 2.66万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780287/
• 关键词: 再生医療; Olfactory Ensheathing Glia(OEG); 鼻粘膜; OEG; 椎間板ヘルニア; 脊髄再生; 脊髄損傷

The main purpose of this study was a clinical trial of spinal cord regeneration therapy by transplanting autologous olfactory ensheathing cells(OECs) derived from nasal mucosa in dogs with severe spinal cord injury(SCI). Although few cases cleared the case selection for OECs transplantation therapy, the owners of dogs did not consent the therapy. However it has been revealed that measurement of concentration of specific protein within cerebrospinal fluid obtained from SCI dogs is useful to assess severity of SCI and predict the outcome. Furthermore, intraoperative somatosensory evoked potential(SEP) could be measured using an extradural catheter electrode in canine SCI cases. The assessment of intraoperative SEP might be used as not only intraoperative monitoring but also predict factor of outcome. In addition, it has been revealed that motor evoked potential(MEP) could be easily measured via skin in dog.

本研究的主要目的是通过移植来自鼻粘膜的自体嗅鞘细胞（OECs）治疗严重脊髓损伤（SCI）的狗的脊髓再生的临床试验。虽然很少有病例明确了嗅鞘细胞移植治疗的病例选择，但狗的主人不同意治疗。然而，已经揭示，从SCI狗获得的脑脊液中的特定蛋白质的浓度的测量是有用的，以评估SCI的严重程度和预测结果。此外，在犬SCI病例中，可以使用硬膜外导管电极测量术中体感诱发电位（SEP）。术中SEP的测定不仅可作为术中监护，而且可作为预后的预测因素。此外，还发现狗的运动诱发电位（MEP）可以很容易地通过皮肤测量。

项目成果

犬の脊髄疾患28例における脳脊髄液中MBPならびにNSEの解析

28例犬脊髓疾病脑脊液MBP和NSE分析

• 发表时间: 2011
• 作者: Takano T;Katoh Y;Doki T;Hohdatsu T;柿崎竹彦;伊藤大介
• 通讯作者: 伊藤大介

Spinal cord injury : New developments

脊髓损伤：新进展

• 发表时间: 2012
• 作者: 柿崎竹彦;山本崇史;和田成一;伊藤伸彦;Ito D
• 通讯作者: Ito D

Myelin basic protein and neuron specific enolase concentration in cerebrospinal fluid in 33 dogs with spinal cord disease

33例脊髓疾病犬脑脊液中髓磷脂碱性蛋白和神经元特异性烯醇化酶浓度

• 发表时间: 2011
• 作者: 柿崎竹彦、大島毅、和田成一、岡野昇三;嶋本良則、村岡登、伊藤伸彦;伊藤大介
• 通讯作者: 伊藤大介

犬の脊髄疾患28例における脳脊髄液中Myelin basic proteinならびにNeuron specific enolaseの解析

28例犬脊髓病脑脊液髓磷脂碱性蛋白及神经元特异性烯醇化酶分析

• 发表时间: 2011
• 作者: Takano T;Ohyama T;Kokumoto A;Satoh R; Hohdatsu T;伊藤大介
• 通讯作者: 伊藤大介