Establishment of a laticifer-specific gene expression system in Hevea brasiliensis

从橡胶树胶乳中获得的天然橡胶具有优异的物理性能，特别是轮胎制造所需的物理性能。为满足天然橡胶不断增长的需求，对H.巴西橡胶是提高天然橡胶产量所必需的，这是通过天然橡胶生物合成中涉及的基因的胶乳特异性过表达实现的。在本研究中，我们成功地发现了胶乳特异性基因，并克隆了这些胶乳特异性基因的5 '上游区域。此外，我们还确定了几个胶乳特异性转录因子，预计参与调控胶乳特异性基因的表达。

Natural rubber obtained from latex in Hevea brasiliensis has the excellent physical properties, especially required for tire manufacturing. To meet the continuously increasing demand for natural rubber, metabolic engineering of H. brasiliensis is required to improve natural rubber production, achieved by a latex-specific overexpression of genes involved in the natural rubber biosynthesis. In this study, we have succeeded in discovering of latex-specific genes and cloning of 5'-upstream regions of these latex specific genes. Furthermore, we also identified several latex-specific transcription factors, expected to participate in the regulation of the latex-specific gene expression.