Identification of the mechanisms of pulmonary surfactant phospholipid transport and lamellar body production.

肺表面活性剂磷脂转运和层状体产生机制的鉴定。

• 批准号: 22790270
• 负责人: HISHIKAWA Daisuke
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790270/
• 关键词: 生体分子医学; 肺サーファクタント; リン脂質移送; 肺胞2型上皮細胞; リン脂質輸送

In this study, I identified the candidate gene "A" for pulmonary surfactant phospholipid transporter. Gene "A" was highly expressed in mouse lung, especially in alveolar type II(ATII) cells, which produce the pulmonary surfactant. Furthermore, the expression of gene "A" was promoted during perinatal periods in mouse lung. The candidate "A" was localized at lamellar bodies in mouse primary ATII cells. The recombinant protein "A" was GTP-dependently bound to the disaturated phosphatidylcholine, which is the major component of pulmonary surfactant phospholipid.

在这项研究中，我确定了肺表面活性物质磷脂转运蛋白的候选基因“A”。基因“A”在小鼠肺中高度表达，特别是在产生肺表面活性物质的肺泡II型（ATII）细胞中。此外，基因“A”的表达在围产期小鼠肺中被促进。候选物“A”定位于小鼠原代ATII细胞中的板层体。重组蛋白“A”是GTP依赖性结合的二饱和磷脂酰胆碱，这是肺表面活性物质磷脂的主要成分。

项目成果

Discovery of lysophospholipid acyltransferases in theLands'cycle

陆地循环中溶血磷脂酰基转移酶的发现

• 发表时间: 2012
• 作者: S.Yamashita;Y.Ishihara;H.Morita;J.Uchiyama;K.Takeuchi;M.Inoue;M.Hirama;進藤英雄
• 通讯作者: 進藤英雄