Identification of the mechanisms of pulmonary surfactant phospholipid transport and lamellar body production.

在本研究中，我确定了肺表面活性物质磷脂转运蛋白的候选基因“A”。基因“A”在小鼠肺中高度表达，特别是在肺泡II型（ATII）细胞中，肺泡II型细胞产生肺表面活性剂。此外，“A”基因的表达在围产期小鼠肺中得到促进。候选“A”定位于小鼠原代ATII细胞的板层体。重组蛋白“A”与不饱和磷脂酰胆碱（肺表面活性剂磷脂的主要成分）有gtp依赖性结合。

In this study, I identified the candidate gene "A" for pulmonary surfactant phospholipid transporter. Gene "A" was highly expressed in mouse lung, especially in alveolar type II(ATII) cells, which produce the pulmonary surfactant. Furthermore, the expression of gene "A" was promoted during perinatal periods in mouse lung. The candidate "A" was localized at lamellar bodies in mouse primary ATII cells. The recombinant protein "A" was GTP-dependently bound to the disaturated phosphatidylcholine, which is the major component of pulmonary surfactant phospholipid.