Evaluation of the mechanism for the release of HMGN1, an alarmin, from LPS stimulated cells.

评估 LPS 刺激的细胞释放 HMGN1（一种警报素）的机制。

• 批准号: 22790416
• 负责人: MURAKAMI Taisuke
• 金额: $ 2.16万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790416/
• 关键词: 敗血症; alarmin; 感染免疫; リポ多糖(LPS); HMGN1; Alarmin

HMGN1, a non-histon chromosomal protein, induces the recruitment of dendritic cells into the sites of HMGN1 administration, and enhances the antigen-specific immune responses. Thus, HMGN1 is estimated to act as an alarmin, when released in the extracellular milieu. In this study, we elucidated the mechanism of HMGN1 release from mouse macrophage RAW cells by LPS stimulation. HMGN1 release was accompanied with cell death which is not affected by Ac-YVAD-CHO, a specific inhibitor of caspase-1. Together these observations indicate that HMGN1 release is accompanied with the LPS-induced cell death, which is independent of caspase-1 activation.

HMGN 1是一种非组蛋白的染色体蛋白，可诱导树突状细胞募集到HMGN 1给药部位，并增强抗原特异性免疫应答。因此，估计HMGN 1在细胞外环境中释放时充当报警蛋白。在这项研究中，我们阐明了HMGN 1从小鼠巨噬细胞RAW细胞通过LPS刺激释放的机制。HMGN 1的释放伴随着细胞死亡，而细胞死亡不受caspase-1特异性抑制剂Ac-YVAD-CHO的影响。总之，这些观察结果表明，HMGN 1的释放伴随着LPS诱导的细胞死亡，这是独立的半胱天冬酶-1激活。

项目成果

Suppressive actions of resolvin D1 on the production and release of septic mediators in D-galactosamine-sensitized endotoxin shock mice

Resolvin D1 对 D-半乳糖胺致敏内毒素休克小鼠脓毒症介质产生和释放的抑制作用

• 发表时间: 2011
• 期刊: Exp Ther Med
• 影响因子: 2.7
• 作者: Murakami. T;Suzuki K;Tamura H;Nagaoka I
• 通讯作者: Nagaoka I

The effect of resolvin D1 on D-galactosamine-primed endotoxin shock model in mice

Resolvin D1对D-氨基半乳糖引发的小鼠内毒素休克模型的影响

• 发表时间: 2010
• 作者: Shimizu;T.;Kida;Y.;Kuwano;K.;村上泰介
• 通讯作者: 村上泰介

Evaluation of the mechanism for the release of HMGN1, an alarmin, from LPS stimulated RAW264.7 cells.日本細菌学会雑誌67 : 156

LPS 刺激的 RAW264.7 细胞释放 HMGN1（一种警报素）的机制评估日本细菌学会杂志 67：156。

• 发表时间: 2012
• 作者: Murakami T;Tamura H;Nagaoka I
• 通讯作者: Nagaoka I

Evaluation of the mechanism for the release of HMGN1, an alarmin, from LPS stimulated RAW264.7 cells

评估 LPS 刺激的 RAW264.7 细胞释放 HMGN1（一种报警素）的机制

• 发表时间: 2012
• 作者: Murakami. T;Suzuki K;Tamura H;Nagaoka I;村上泰介
• 通讯作者: 村上泰介

An antimicrobial cathelicidin peptide LL-37 suppresses the LPS-induced endothelial cell apoptosis in vitro and in vivo

抗菌抗菌素肽 LL-37 在体外和体内抑制 LPS 诱导的内皮细胞凋亡

• 发表时间: 2011
• 作者: Suzuki K;Murakami T;Tamura H;Nagaoka I
• 通讯作者: Nagaoka I