Foundation of alveolar bone regenerative therapy strategy used new synthetic peptide

新型合成肽牙槽骨再生治疗策略的基础

• 批准号: 22592324
• 负责人: UEDA Masatoshi
• 金额: $ 2.83万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592324/
• 关键词: エナメル基質タンパク; 歯根膜細胞; 歯周組織再生; エナメルマトリックスデリバティブ; 合成ペプチド; 骨髄細胞; 硬組織分化誘導; エナメルマトリックス; 歯周病; 再生治療; ペプチド

One method of treating periodontitis is to use EmdogainR, a material derived from the tooth germ of juvenile swine that promotes periodontal tissue regeneration, including the formation of hard tissue as cementum, alveolar bone. The use of EmdogainR is therefore established in the field of periodontal regenerative therapy. However, because of its swine origin, some patients choose not to be treated with EmdogainR. The active component of EmdogainR has been shown to be a peptide whose sequence corresponds to an amelogenin II precursor. As such, this peptide may function as a growth factor to stimulate cell differentiation and tissue regeneration. In this study, we characterized the effects of the synthetic EmdogainR-derived peptide on the proliferation, adhesion, migration and differentiation of periodontal ligament fibroblasts (HPdLFs), which display properties similar to mesenchymal stem cells, and Sprague-Dawley rat bone marrow (RBM) cells. Compared to cells not treated with tesynthetic EmdogainR-derived peptide, treated cells showed increased proliferation, initial adhesion, and chemotactic activity. The optimum peptide concentration that stimulated these activities was determined to be 100 ng/ml. We next investigated the effects of the peptide at a concentration of 100 ng/ml on osteogenesis and cementogenesis in HPdLF cells by assaying alkaline phosphatase activity, osteocalcin production, and mineralization. Taken together, our data suggest that the EmdogainR-derived peptide stimulates periodontal hard tissue regeneration by stimulating the proliferation, adhesion, and migration of mesenchymal stem cells.

治疗牙周炎的一种方法是使用EmdogainR，这是一种来源于幼年猪牙胚的材料，可促进牙周组织再生，包括牙骨质、牙槽骨等硬组织的形成。因此，在牙周再生治疗领域确立了EmdogainR的用途。然而，由于其来源于猪，一些患者选择不接受EmdogainR治疗。EmdogainR的活性成分已被证明是一种肽，其序列对应于釉原蛋白II前体。因此，该肽可以作为生长因子发挥作用以刺激细胞分化和组织再生。在这项研究中，我们表征了合成的EmdogainR衍生肽对牙周膜成纤维细胞（HPdLFs）和Sprague-Dawley大鼠骨髓（RBM）细胞的增殖、粘附、迁移和分化的影响，HPdLFs显示出与间充质干细胞相似的特性。与未用合成的EmdogainR衍生肽处理的细胞相比，处理的细胞显示出增加的增殖、初始粘附和趋化活性。刺激这些活性的最佳肽浓度确定为100 ng/ml。接下来，我们通过测定碱性磷酸酶活性、骨钙素产生和矿化来研究浓度为100 ng/ml的肽对HPdLF细胞中骨生成和牙骨质生成的影响。综上所述，我们的数据表明，EmdogainR衍生肽刺激牙周硬组织再生刺激间充质干细胞的增殖，粘附和迁移。

项目成果

ラット骨髄細胞に対するエナメル基質タンパク由来合成ペプチドの初期硬組織分化誘導

使用源自大鼠骨髓细胞牙釉质基质蛋白的合成肽诱导早期硬组织分化

• 发表时间: 2011
• 作者: Harada K;Matsubara T;Nakamura N;安井菜津希,田口洋一郎,富永和也,高橋宰達,奥田麻貴子,木村大輔,片山暢仁,南堂百映,田中昭男,上田雅俊
• 通讯作者: 安井菜津希,田口洋一郎,富永和也,高橋宰達,奥田麻貴子,木村大輔,片山暢仁,南堂百映,田中昭男,上田雅俊

In vitro effects of EMD-derived oligopeptides on human epithelial cells

EMD 衍生寡肽对人上皮细胞的体外影响

• 发表时间: 2011
• 作者: Natsuki Yasui;Yoichiro Taguchi;Kazuya Tominaga;Yoshiya Hashimoto;Shoichiro Terada;Nobuhiro Shigematsu;Shoji Terada;Akio Tanaka;Masatoshi Ueda
• 通讯作者: Masatoshi Ueda

Biological Effects of EmdogainR-derived Oligopeptideson Rat Bone Marrow Cells in Vitro

EmdogainR 衍生寡肽对大鼠骨髓细胞的体外生物学效应

• 发表时间: 2012
• 期刊: Journal of Oral Tissue Engineering
• 作者: Natsuki Yasui;Yoichiro Taguchi;Akio Tanaka;Masatoshi Ueda and Makoto Umeda.
• 通讯作者: Masatoshi Ueda and Makoto Umeda.