Development of the general tags for formation of hetero-complex

为了开发能够控制和促进异源复合物形成的通用标签，HA-标签，我们首先从PDB的蛋白质晶体结构（超过3A分辨率）中通过计算机搜索标签的候选肽。最终选择了两种异源二聚体标签（2 HA-tag）。我们构建了在N端或C端融合有2 HA标签的模型蛋白，并在大肠杆菌中过量表达。异源二聚体形成的实验正在进行中。

In order to develop the general tags, HA-tags which can control and promote the formation of a hetero complex, we first searched candidate peptides of the tag from the protein crystal structures(more than the 3A resolution) from PDB in silico. Finally two kinds of hetero-dimer tag(2HA-tag) were selected. We constructed model proteins with 2HA-tag fusion at the N or C terminal, and over-expressed them in Escherichia coli. The experiments of hetero-dimer formation are in progress.