Development of the general tags for formation of hetero-complex

用于形成异质复合物的通用标签的开发

• 批准号: 22657028
• 负责人: YAO Min
• 金额: $ 2.25万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657028/
• 关键词: X線結晶解析; 蛋白質工学; 遺伝子工学; タグ; ヘテロ複合体; 多量体形; バイオインフォマティクス

In order to develop the general tags, HA-tags which can control and promote the formation of a hetero complex, we first searched candidate peptides of the tag from the protein crystal structures(more than the 3A resolution) from PDB in silico. Finally two kinds of hetero-dimer tag(2HA-tag) were selected. We constructed model proteins with 2HA-tag fusion at the N or C terminal, and over-expressed them in Escherichia coli. The experiments of hetero-dimer formation are in progress.

为了开发能够控制和促进异源复合物形成的通用标签HA标签，我们首先从PDB in silico的蛋白质晶体结构（超过3A分辨率）中搜索标签的候选肽。最终选择了两种异源二聚体标签(2HA-tag)。我们构建了 N 端或 C 端带有 2HA 标签融合的模型蛋白，并在大肠杆菌中过表达它们。异源二聚体形成的实验正在进行中。

项目成果

Preliminary X-ray crystallographic study of the receptor-binding domain of the D/C mosaic neurotoxin from Clostridium botulinum

肉毒杆菌 D/C 嵌合神经毒素受体结合域的 X 射线晶体学初步研究

• 发表时间: 2010
• 期刊: Acta Crystallogr Sect F Struct Biol Cryst Commun
• 作者: Nuemket N.;Tanaka Y.;Tsukamoto K.;Tsuji T.;Nakamura K.;Kozaki S.;Yao M.;Tanaka I.
• 通讯作者: Tanaka I.

Crystallization and preliminary X-ray crystallographic analysis of eIF5BΔN and the eIF5BΔN-eIF1AΔN complex

eIF5BΔN 和 eIF5BΔN-eIF1AΔN 复合物的结晶和初步 X 射线晶体学分析

• DOI: 10.1107/s1744309111015910
• 发表时间: 2011
• 期刊: Acta Cryst.
• 作者: Aiping Zheng;Reo Yamamoto;Masaaki Sokabe;Isao Tanaka and Min Yao
• 通讯作者: Isao Tanaka and Min Yao

Crystallization and preliminary X-ray studies of azoreductases from Bacillus sp. B29.

• DOI: 10.1107/s1744309110007785
• 发表时间: 2010-05
• 期刊: Acta crystallographica. Section F, Structural biology and crystallization communications
• 作者: D. Ogata;T. Ooi;T. Fujiwara;S. Taguchi;I. Tanaka;M. Yao