Establishment of rapid detection system for intra-amniotic infection and drug susceptibility test by PCR using eukaryote-made Taq polymerase

真核生物Taq聚合酶PCR羊膜内感染快速检测体系的建立及药敏试验

• 批准号: 22659297
• 负责人: SAITO Shigeru
• 金额: $ 1.89万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22659297/
• 关键词: 絨毛膜羊膜炎; 早産; 感染; PCR; Ureaplasma; Mycoplasma; 真菌; 羊水; 細菌; 慢性肺疾患; 病原微生物; 迅速診断法; Universal PCR; 16SrRNA; 産科学; 検査・診断システム; 子宮内感染; 病原体検出; 薬剤感受性試験

We have established a novel method to detect bacterial, Mycoplasma, Ureaplasma and fungi in the amniotic fluid using eukaryote-made Taq polymerase. Using this system, we have evaluated the bacterial infection in amniotic fluid of 86 preterm labor cases. Surprisingly, we have detected double infection with bacterial Ureaplasma in 13 case, Ureaplasma infection cases in 5 cases, bacterial infection cases in 13 cases, and infection rate in preterm labor cases accounted for 37.2%. In double infection cases, amniotic fluid IL-8 was very high （mean: 129.5ng/ml), and frequency of chronic lung disease was also very high （38.5%) compared to those in non-infected cases （5.6%). The duration from the amniocentasis to delivery in double infection cases was significantly shorter than that in no infection cases.

我们已经建立了一种新的方法，可以使用真核生物制造的TAQ聚合酶在羊水中检测细菌，支原体，尿素和真菌。使用该系统，我们评估了86例早产案例的羊水中的细菌感染。令人惊讶的是，我们在13例病例中检测到了细菌尿素超倍年的双重感染，5例尿素倍率感染，13例的纤维化感染病例，早产劳动病例的感染率为37.2％。在双感染病例中，羊水IL-8非常高（平均：129.5ng/ml），与未感染病例相比，慢性肺病的频率也很高（38.5％）（38.5％）（5.6％）。在双重感染病例中输送的羊膜中的持续时间比在没有感染病例的情况下要短得多。

项目成果

Distribution of serine/threonine kinase SAD-B in mouse peripheral nerve synapse

• DOI: 10.1097/wnr.0b013e328346013c
• 发表时间: 2011-05-11
• 期刊: NEUROREPORT
• 影响因子: 1.7
• 作者: Hagiwara, Akari;Harada, Kenu;Ohtsuka, Toshihisa
• 通讯作者: Ohtsuka, Toshihisa

Prickle2 is localized in the postsynaptic density and interacts with PSD-95 and NMDA receptors in the brain

• DOI: 10.1093/jb/mvr023
• 发表时间: 2011-06-01
• 期刊: JOURNAL OF BIOCHEMISTRY
• 影响因子: 2.7
• 作者: Hida, Yamato;Fukaya, Masahiro;Ohtsuka, Toshihisa
• 通讯作者: Ohtsuka, Toshihisa

Reduced stathmin-1 expression in NK cells associated with spontaneous abortion.

与自然流产相关的 NK 细胞中 stathmin-1 表达减少。

• 发表时间: 2011
• 期刊: Am J Pathol. 178
• 作者: Lin Y.;Li C.;Shan B.;Wang W.;Saito S.;Xu J.;Di J.;Zhong Y.;Li D.J.
• 通讯作者: Li D.J.

頸管無力症既往妊婦の管理方針について

有宫颈机能不全史孕妇的管理政策

• 发表时间: 2010
• 期刊: 産婦人科の実際
• 作者: 米田哲;米田徳子;齋藤滋
• 通讯作者: 齋藤滋

Regulatory-T cells and -NK cells play important roles for maintenance of pregnancy.

调节性 T 细胞和 NK 细胞对于维持妊娠发挥着重要作用。

• 发表时间: 2013
• 作者: Kitaoka Y;他4名;Saito S
• 通讯作者: Saito S