MicroRNA Targeting of Oncolytic Viruses for cancer therapy

用于癌症治疗的溶瘤病毒的 MicroRNA 靶向

• 批准号: 22680065
• 负责人: NAKAMURA Takafumi
• 金额: $ 9.24万
• 依托单位: Tottori University (2011)The University of Tokyo (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22680065/
• 关键词: 遺伝子治療; ウイルス療法; 癌; バイオテクノロジー; トランスレーショナルリサーチ

Oncolytic viruses are promising therapeutic agents for cancer and are currently under clinical investigation. The virotherapy is novel strategy that viruses infect and replicate within tumor cells, directly lysing and killing them. We have reported that microRNA (miRNA) regulation enables tumor-specific viral replication by altering the expression of a targeted viral gene of an attenuated vaccinia virus vaccine strain LC16m8. We used miRNA-based gene regulation to suppress B5R expression through let-7a, a miRNA that is downregulated in many tumors. The miRNA-regulated vaccinia virus achieved efficient viral replication and oncolysis in cancer cells, but elimination of unwanted replication and associated toxicity in normal cells in vivo.

溶瘤病毒是一种很有前途的癌症治疗药物，目前正在进行临床研究。病毒治疗是一种新的策略，病毒在肿瘤细胞内感染和复制，直接裂解和杀死它们。我们已经报道，microRNA(MiRNA)调控通过改变减毒痘苗病毒疫苗株LC16m8的靶向病毒基因的表达来实现肿瘤特异性病毒复制。我们使用基于miRNA的基因调控通过let-7a抑制B5R的表达，let-7a是一种在许多肿瘤中下调的miRNA。MiRNA调控的痘苗病毒在癌细胞中实现了有效的病毒复制和溶瘤，但在体内消除了正常细胞中不必要的复制和相关的毒性。

项目成果

Exploitation of the interaction of measles virus fusogenic envelope proteins with the surface receptor CD46 on human cells for microcell-mediated chromosome transfer.

• DOI: 10.1186/1472-6750-10-37
• 发表时间: 2010-05-06
• 期刊: BMC biotechnology
• 影响因子: 3.5
• 作者: Katoh M;Kazuki Y;Kazuki K;Kajitani N;Takiguchi M;Nakayama Y;Nakamura T;Oshimura M
• 通讯作者: Oshimura M

マイクロRNAによって制御されるウイルスの開発とその応用

microRNA控制病毒的研制及其应用

• 发表时间: 2010
• 作者: Sawada Y;Sugita K;Kabashima R;Hino R;Nakamura M;Koga C;Tokura Y;高橋昭久;中村貴史
• 通讯作者: 中村貴史

MicroRNA Targeting of Oncolytic Viruses for cancer therapy

用于癌症治疗的溶瘤病毒的 MicroRNA 靶向

• 发表时间: 2011
• 作者: Takafumi Nakamura.

Highly Attenuated Vaccinia Virus as a Potential Oncolytic Agent for Cancer Virotherapy

高度减毒痘苗病毒作为癌症病毒治疗的潜在溶瘤剂

• 发表时间: 2010
• 作者: Fusasaki T;Narita R;Hiura M;Abe S;Tabaru A;Hino R;Matsuyama A;Shimajiri S;Tokura Y;Sasaguri Y;Harada M;Nakamura T
• 通讯作者: Nakamura T

MicroRNA-regulated oncolytic vaccinia virus not only enhances the oncolytic activity but also reduces the viral pathogenicity

MicroRNA调控的溶瘤痘苗病毒不仅增强溶瘤活性还降低病毒致病性

• 发表时间: 2010
• 作者: Nakamura M;Sugita K;Tokura Y;大西武雄;Nakamura T
• 通讯作者: Nakamura T