Development of anti-cancer agents targeting the endocytic pathway

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The purpose of this study is to construct a cell morphology-based high-content screening system and to develop anti-cancer agents targeting the endocytic pathway. We constructed a chemical-genetic phenotype profiling system based on the high-content cell morphology database, Morphobase. We showed that NPD1801, which perturbs the endocytic pathway, targeted calmodulin, caused the activation of Ras-Raf pathway, the inhibition of the fusion of late endosome and lysosome, and the dysfunction of lysosome, and resulted in the cancer cell death.