Characterization of Muse cells, non-tumorigenic intrinsic pluripotent stem cells, and their application to regenerative medicine.

MUSE细胞是一种新型的非致瘤多能干细胞，通常存在于人间充质组织中，本项目对其独特的性质进行了研究。将缪斯细胞注入肝肾损伤动物模型的血流中，使其向受损组织中迁移并整合，而在非缪斯细胞中未观察到这种现象。非缪斯细胞不会迁移或整合到受损的肝和肾中，也不会留在正常的完整组织中。进一步观察到，在肝损伤模型中，小鼠细胞自发分化为肝细胞、胆管等肝成分细胞；在卒中模型中，小鼠自发分化为神经细胞，并有助于组织再生和功能恢复。MUSE细胞的这种独特的迁移和整合能力与其细胞表面G蛋白偶联受体的特异性表达密切相关。

The unique property of Muse cells, novel type of non-tumorigenic pluripotent stem cells that normally reside in human mesenchymal tissues, was investigated in this project. Muse cells positively migrated and integrated into the damaged tissue when they were infused into the blood stream of animal models with liver or kidney damages, whereas such phenomenon was not observed in non-Muse cells. Non-Muse cells did not migrate or integrate into damaged liver and kidney nor did they remained in normal intact tissues. Muse cells were further observed to be spontaneously differentiated into hepatocytes, bile ducts and other liver component cells in the liver damage model, and into neural cells in the stroke model, and contributed into tissue regeneration and functional recovery. Such unique property of migration and integration abilities of Muse cells are found to be tightly related to specific expression of G-protein coupled receptor expression on their cell surface.