Molekulare Analyse von Eisen- und Zink-Aufnahme Systemen in Escherichia coli mit Schwerpunkt ZupT

以 ZupT 为重点的大肠杆菌铁和锌吸收系统的分子分析

• 批准号: 5403028
• 负责人: Privatdozent Dr. Gregor Grass
• 金额: --
• 依托单位: Institutsbereich Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5403028?language=en
• 关键词: Molekulare; Analyse; von; Eisen; und

The ZupT protein is the first described member of the ZIP (Zrt- and Irt-related protein) family of iron and zinc transportes in prokaryotes. ZIP-like proteins occur in all domains of life, including man, and are important factors for iron, manganese and zinc homeostasis. The goals of our proposal are (i) to understand the physiological role of ZupT amongst other ferrous transporters in Escherichia coli, (ii) to identify amino acid residues in ZupT important for function and metal specifity, (iii) to characterize ZupT mediated transport biochemically in cooperation with Christopher Rensing (University of Arizona, Tucson, USA) and (iv) to identify new iron transporters employing DNA-micro array analysis for E. coli.

ZupT蛋白是在原核生物中铁和锌转运蛋白的ZIP（Zrt和Irt相关蛋白）家族的第一个被描述的成员。ZIP样蛋白存在于生命的各个领域，包括人类，并且是铁，锰和锌稳态的重要因素。我们计划的目标是（i）了解ZupT在大肠杆菌中其他亚铁转运蛋白中的生理作用，（ii）鉴定ZupT中对功能和金属特异性重要的氨基酸残基，（iii）与Christopher Rensing合作，以生物化学方式表征ZupT介导的转运（University of Arizona，Tucson，USA）和（iv）采用DNA微阵列分析鉴定E.杆菌