Development of an immobilization method for cDNA on an electronic circuit and an application for a detection of mRNA

我们已经建立了一种电化学图案化方法，该方法可以通过安装在电路板上的微电极上的电化学反应来控制DNA或蛋白质分子的固定。在集成了25对200微米的微电极的情况下，在4mm面积上实现了蛋白质的图案化。我们还发现，在试管中使用模板DNA的传统无细胞蛋白质合成方法可以在安装在电路板上的微电极上进行，并且可以检测蛋白质的表达。

We have been established an electrochemical patterning method which is possible to control an immobilization of DNA or protein molecule by an electrochemical reaction on a microelectrode mounted on a circuit board. Patterning of protein on 4mm area has been achieved where 25 pair of microelectrodes of 200 micrometer was integrated. We also found that traditional cell-free protein synthesis method in a test tube using a template DNA could be progressed on a microelectrode mounted on a circuit board and an expression of a protein could be detected.