Bacterial Phenotype Switching via Large-Scale Chromosome Flip-Flop Inversion

通过大规模染色体触发器反转进行细菌表型转换

• 批准号: 23570009
• 负责人: CUI Longzhu
• 金额: $ 3.49万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570009/
• 关键词: ゲノム構築; ゲノム再編; ゲノム維持; ゲノム逆位; 表現型スイッチング; 黄色ブドウ球菌; ヘテロ性質; 持続感染; Small colony variant; Genome inversion; Phenotype switching; Heterogeneity; Genome rearrangement; 薬剤耐性

In this study found that a bacterium that generates a reversible, large-scale inversion of its chromosome (about half of its total genome) at high frequencies of up to once every four generations. This inversion switches on or off bacterial phenotypes, including colony morphology, antibiotic susceptibil- ity, hemolytic activity, and expression of dozens of genes. Quan- titative measurements and mathematical analyses indicate that this reversible switching is stochastic but self-organized so as to maintain two forms of stable cell populations (i.e., small colony variant, normal colony variant) as a bet-hedging strategy. Thus, this heritable and reversible genome fluctuation seems to govern the bacterial life cycle; it has a profound impact on the course and outcomes of bacterial infections.

在这项研究中发现，一种细菌以高达每四代一次的高频率产生可逆的、大规模的染色体反转（约占其总基因组的一半）。这种倒置可以打开或关闭细菌表型，包括菌落形态、抗生素敏感性、溶血活性和数十种基因的表达。定量测量和数学分析表明，这种可逆切换是随机的，但也是自组织的，从而维持两种形式的稳定细胞群体（即小群体变异，正常群体变异）作为一种下注对冲策略。因此，这种可遗传和可逆的基因组波动似乎控制着细菌的生命周期；它对细菌感染的过程和结果有深远的影响。

项目成果

Staphylococcal Phenotype Switching via a Large-scale Genome Flip-Flop

通过大规模基因组触发器进行葡萄球菌表型转换

• 发表时间: 2012
• 作者: 小内清;石浦正寛;河合久仁子・近藤憲久・ビクトル ボイコ・大泰司紀之;Longzhu Cui
• 通讯作者: Longzhu Cui

Longzhu cui, et al

崔龙珠等

• 发表时间: 2012
• 期刊: Proc Natl Acad Sci USA
• 作者: Longzhu cui;et al
• 通讯作者: et al

Reversible Chromosome Inversion and Bacterial Heterogeneous Population

可逆染色体倒位和细菌异质群

• 发表时间: 2012
• 作者: Longzhu Cui;Hui-min Neoh;Akira Iwamoto and Keiichi Hiramatsu
• 通讯作者: Akira Iwamoto and Keiichi Hiramatsu

Reversible Chromosome Inversion and Phenotype Switching of Staphylococcal SCV

葡萄球菌 SCV 的可逆染色体倒转和表型转换

• 发表时间: 2013
• 作者: Longzhu Cui;Akira Iwamoto;Hiramatsu Keiichi and Hideaki Hanaki
• 通讯作者: Hiramatsu Keiichi and Hideaki Hanaki

Staphylococcal Phenotype Switching via a Large-scale Genome Flip-Flop Inversion

通过大规模基因组触发器反转进行葡萄球菌表型转换

• 发表时间: 2012
• 作者: Eguchi;K.;Yoshioka;Y.;Yoshida;H.;Morishita;K.;Miyata;S.;Hiai;H. and Yamaguchi;M.;Longzhu Cui
• 通讯作者: Longzhu Cui