Research on active compounds from natural resources affecting the differentiation of ES and iPS cells

自然资源中影响 ES 和 iPS 细胞分化的活性化合物的研究

• 批准号: 23590026
• 负责人: ITO CHIHIRO
• 金额: $ 3.33万
• 依托单位: Meijo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590026/
• 关键词: 天然資源; アポトーシス誘導活性; ヒスタミン放出抑制活性; がん細胞増殖抑制活性; 抗酸化活性; NO産生抑制活性; ES・iPS細胞分化; キノリン-2,4-ジオンアルカロイド; ビフェニル誘導体; ベンゾフェノン; 細胞毒性; カルバゾールキノン誘導体; NO 産生抑制活性; カルバゾールアルカロイド; ケイヒ酸誘導体; ロテノイド誘導体; マイヤーレモン; 機能性成分; Citrus tachibana; フラボノイド; クマリン; 肥厚性瘢痕由来線維芽細

Several new compounds were isolated from plant sources together with known compounds. The structures of the new compounds were elucidated by spectroscopic analyses. Several of the isolated compounds exhibited the following useful biological activities: severibuxine, murrayafoline-A, and murrayazolinine significantly suppressed the growth of HL-60 leukemia cells by causing apoptosis; lansamide I, lansiumamide B, and SB-204900 significantly decreased histamine release from RBL-2H3 cells; and lapachol decreased the proliferation rate of hypertrophic scar fibroblasts. Aromatic compounds produced by Eurotium herbariorum from a karebushi were isolated; of these, several benzaldehyde derivatives exhibited high radical scavenging activity. Three carbazolequinone derivatives, synthesized using murrayaquinone-A as a lead compound, inhibited LPS/IFN-induced NO production. No seed compounds affecting the differentiation of ES and iPS cells have been found; however, future research is planned.

从植物中分离出几种新化合物和已知化合物。新化合物的结构经波谱分析鉴定。几种分离的化合物表现出以下有用的生物活性：severibuxine、murrayafoline-A和murrayazolinine通过引起细胞凋亡显著抑制HL-60白血病细胞的生长; lansamide I、lansiumamide B和SB-204900显著降低RBL-2 H3细胞的组胺释放;拉帕胆碱降低增生性瘢痕成纤维细胞的增殖率。从karebushi散囊菌产生的芳香族化合物进行了分离，其中，几个苯甲醛衍生物表现出较高的自由基清除活性。三个咔唑醌衍生物，使用Murrayaquinone-A作为先导化合物合成，抑制LPS/IFN诱导的NO产生。没有发现种子化合物影响ES和iPS细胞的分化;然而，未来的研究正在计划中。

项目成果

Inhibitory effect of carbazolequinone derivatives on lipopolysaccharide and interferon-γ- induced nitric oxide production in mouse macrophage RAW264.7 cells

咔唑醌衍生物对脂多糖和干扰素-γ诱导的小鼠巨噬细胞RAW264.7细胞一氧化氮产生的抑制作用

• 发表时间: 2013
• 期刊: J. Pharm. Pharmacol
• 作者: Tomiyasu Murata;Susumu Kohno;Chihiro Ito;Masataka Itoigawa;Ayumi Sugiura;Kiyomi Hikita;Norio Kaneda
• 通讯作者: Norio Kaneda

Apoptosis of HL-60 leukemia cells induced by carbazole alkaloids isolated from Murraya euchrestifolia

九里香咔唑生物碱诱导HL-60白血病细胞凋亡

• 发表时间: 2012
• 期刊: J. Nat. Med
• 作者: Chihiro Ito;Masataka Itoigawa;Keisuke Nakao;Tomiyasu Murata;Norio Kaneda;Hiroshi Furukawa
• 通讯作者: Hiroshi Furukawa

Natsudaidain isolated from Citrus plants inhibits activation of mast cells in the late phase of immediate allergy

从柑橘类植物中分离出的 Natsudaidain 可抑制即时过敏后期肥大细胞的活化

• 发表时间: 2013
• 作者: Takuya Matsui;Hiroyuki Shiono;Chihiro Ito;Masataka Itoigawa;Tadashi Okada
• 通讯作者: Tadashi Okada

Flavonoid, Phenylpropanoid and Coumarins Characteristics in Lemons from the Bonin Islands (Kikuchi Lemons)

小笠原群岛柠檬（菊池柠檬）中的类黄酮、苯丙素和香豆素特征

• 发表时间: 2013
• 期刊: Nippon Shokuhin Kagaku Kogaku Kaishi
• 作者: Yoshiaki Miyake;Chihiro Ito;Masataka Itoigawa
• 通讯作者: Masataka Itoigawa

ミカン科Acronychia pedunculataの成分研究

芸香科植物花肩甲的成分研究

• 发表时间: 2012
• 作者: 井藤千裕;細井麻里;糸魚川政孝;呉天賞
• 通讯作者: 呉天賞