Role of miRNAs in the development of bronchial smooth muscle hyperresponsiveness in allergic asthma

miRNA 在过敏性哮喘支气管平滑肌高反应性发展中的作用

• 批准号: 23590118
• 负责人: CHIBA Yoshihiko
• 金额: $ 3.41万
• 依托单位: Hoshi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590118/
• 关键词: アレルギー性気管支喘息; 気道過敏性; 気管支平滑筋; microRNA (miRNA); miR-140; RhoA; CD38; Interleukin-13 (IL-13); miR-140-5p; TLR4; サイトカイン; interleukin-13 (IL-13)

Current study was carried out to make clear the mechanism(s) of development of airway hyperresponsiveness in asthmatics. In bronchial smooth muscles of mice with allergic asthma, an up-regulation of RhoA protein and a down-regulation of miR-140-3p were observed. In cultured human bronchial smooth muscle cells, an up-regulation of RhoA protein was observed when the function of endogenous miR-140-3p was inhibited by its antagomir. Treatment of the cells with interleukin-13 caused an up-regulation of RhoA protein and a down-regulation of miR-140-3p. These findings suggest that RhoA protein expression is negatively regulated by miR-140-3p in bronchial smooth muscle cells. The increased expression of interleukin-13 in the airways of asthmatics might, at least in part, contribute to the reduction of miR-140-3p, resulting in an up-regulation of RhoA protein and augmented smooth muscle contraction of the airways.

本研究旨在阐明哮喘患者气道高反应性的发生机制(S)。在过敏性哮喘小鼠的支气管平滑肌中，RhoA蛋白表达上调，miR-140-3p表达下调。在培养的人支气管平滑肌细胞中，当内源性miR-140-3p的功能被其拮抗剂抑制时，RhoA蛋白表达上调。经IL-13处理后，RhoA蛋白表达上调，miR-140-3p表达下调。这些发现表明，MIR-140-3p对RhoA蛋白的表达具有负性调节作用。哮喘患者呼吸道IL-13的表达增加，可能至少部分参与了miR-140-3p的表达减少，导致RhoA蛋白表达上调，增强了呼吸道的平滑肌收缩。

项目成果

Downregulation of miR-140-3p Contributes to Upregulation of CD38 Protein in Bronchial Smooth Muscle Cells.

• DOI: 10.3390/ijms21217982
• 发表时间: 2020-10-27
• 期刊: International journal of molecular sciences
• 影响因子: 5.6
• 作者: Chiba Y;Matsumoto M;Hanazaki M;Sakai H
• 通讯作者: Sakai H

Different effects of smoke from heavy and light cigarettes on induction of bronchial smooth muscle hyperresponsiveness in rats

浓烟和淡烟烟雾对诱导大鼠支气管平滑肌高反应性的不同影响

• 发表时间: 2011
• 期刊: J. Smooth Muscle Res
• 作者: Sakai;H.;Fujita;A.;Watanabe;A.;Chiba;Y.;Kamei;J. and Misawa;M
• 通讯作者: M

アレルギー性気管支喘息時の気管支平滑筋 TLR4 up-regulation における miR-140-5p の役割

miR-140-5p在过敏性支气管哮喘期间支气管平滑肌TLR4上调中的作用

• 作者: 千葉義彦;中原孝史;清水一樹;須藤怜奈;須藤 航;田上 元;團野駿太;加藤靖菜;安藤桂子;松本麻友美;酒井寛泰;成田 年;花崎元彦;片山 浩
• 通讯作者: 片山 浩

Elevated guanylate cyclase and cyclic-guanosine monophosphate-dependent protein kinase levels in nasal mucosae of antigen-challenged rats.

• DOI: 10.1016/j.mvr.2013.08.009
• 发表时间: 2013-11
• 期刊: Microvascular research
• 影响因子: 3.1
• 作者: Hiroyasu Sakai;T. Hara;Kenji Todoroki;Yuma Igarashi;M. Misawa;M. Narita;Y. Chiba

Involvement of the Tyr kinase/JNK pathway in carbachol-induced bronchial smooth muscle contraction in the rat

Tyr 激酶/JNK 通路参与卡巴胆碱诱导的大鼠支气管平滑肌收缩

• 发表时间: 2013
• 期刊: Anesthesiology
• 影响因子: 8.8
• 作者: Sakai;H.;Watanabe;Y.;Honda;M.;Tsuiki;R. Ueda;Y.;Nagai;Y.;Narita;M. and Chiba;Y
• 通讯作者: Y