In vitro approaches to evaluate placental drug transport by using differentiating JEG-3 human choriocarcinoma cells

人绒毛膜癌细胞已被用作研究胎盘滋养细胞跨细胞药物转运的模型。然而，这些模型允许低分子量药物通过细胞间隙连接进行运输。本研究旨在探讨JEG-3绒毛膜癌细胞在不同培养条件下的分化模式，建立合适的体外合胞滋养细胞药物转运模型。体外培养的JEG-3的分化模式与体内的合体滋养细胞样分化信号特征相似。综上所述，CS-C培养的JEG-3细胞分化的合体滋养细胞样模型可能适合于评估药物在胎盘滋养细胞中的转运。

Human choriocarcinoma cells have been used as models for studying transcellular drug transport through placental trophoblasts. However, these models allow the transport of low-molecular-weight drugs through intercellular gap junctions. This study aimed to investigate the differentiation patterns of JEG-3 choriocarcinoma cells under different culture conditions and establish the appropriate model of in vitro syncytiotrophoblast drug transport. The differentiation pattern of JEG-3 cultured in CS-C resembled the syncytiotrophoblast-like differentiation signal characterizations in vivo. In conclusion, the syncytiotrophoblast-like models of differentiating JEG-3 cells cultured in CS-C might be appropriate for evaluating drug transport across the placental trophoblast.