A study of toxic effect of amyloid substance in brain

淀粉样物质脑毒性作用的研究

• 批准号: 04670716
• 负责人: IKEDA Kenji
• 金额: $ 0.9万
• 依托单位: Tokyo Institute of Psychiatry
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670716/
• 关键词: amyloidsubstance; toxic effect; neurofibrillary tangle; preamyloid; アルツハイマー病; アミロイド; 老人斑; 免疫組織化学; タウタンパク; 微細構造

Toxic effect of amyloid substances against tissue was investigated. Diffuse type of senile plaques are thought to be an initial step of amyloid-formation. beta-amyloid or preamyloid in diffuse plaque was observed by electron microscopy applying immunohistochemical and methemanine-silver impregnation methods. The amyloid substance in this structure consisted of fine short fibrillar bundle and was partially amorphic. Most of the preamyloid deposited between small processes. The membrane of these processes showed unclear appearance in comparison to the same structure in other portion without preamyloid. This finding may indicate that the preamyloid induce the degeneration of the surrounding tissue due to its toxic effect.Another important abnormal protein with amyloid nature, the neurofibrillary tangles (NFT) , was also investigated to elucidate its toxic effect. Extracellular NFT was, differ from intraneuronal NFT,surrounded by degenerated neuronal processes and invaded by numerous astrocytic processes and microglia. The Immunohistochemical and ultrastructural alteration of extracellular NFT was also noticed. A series of these findings may be the evidence of toxic effect of NFT against the surrounding tissue.

研究了淀粉样物质对组织的毒性作用。弥漫型老年斑被认为是淀粉样蛋白形成的第一步。用免疫组织化学和甲胺-银染色法在电子显微镜下观察弥漫性斑块中的β-淀粉样蛋白或前淀粉样蛋白。该结构中的淀粉样物质由细小的短纤维束组成，部分无定形。大部分前突沉积在小突起之间。与无前叶样突起的其他部分的相同结构相比，这些突起的膜外观不清。这一发现可能表明前叶蛋白由于其毒性作用而导致周围组织的退化。另一种具有淀粉样蛋白性质的重要异常蛋白-神经原纤维缠结(NFT)也被研究以阐明其毒性作用。与神经元内NFT不同，细胞外NFT被变性的神经元突起包围，并被大量的星形细胞突起和小胶质细胞侵犯。细胞外NFT免疫组织化学和超微结构改变。这些发现中的一系列可能是NFT对周围组织产生毒性作用的证据。

项目成果

Ikeda K et al.: "Anti-tau positive fibrillary tangles in the brain of postencephaliticparkinsonism of Economo type" Neuroscience Letters. 162. 176-178 (1993)

Ikeda K 等人：“Economo 型脑炎后帕金森病大脑中的抗 tau 蛋白阳性纤维缠结”《神经科学快报》。

Ikeda K.et al.: "Neuritic degeneration in Alzheimer-type dementia, with special reference to the presence of dark neurites" Brain and Nerve. 44. 449-455 (1992)

Ikeda K.等人：“阿尔茨海默型痴呆中的神经炎变性，特别是暗神经突的存在”《大脑和神经》。

Ikeda K.et al.: "Coexistence of paired helical filaments and glial filaments in astrocytic processes within ghost tangles" Neurosci Lett.148. 126-128 (1992)

Ikeda K.等人：“幽灵缠结内星形胶质细胞过程中成对螺旋丝和神经胶质丝的共存”Neurosci Lett.148。

池田研二 他: "長期の臨床経過を辿り老人斑に乏しいアルツハイマー型老年痴呆の1例" 脳と神経. 45. 455-460 (1993)

Kenji Ikeda 等人：“一例临床病程较长且老年斑很少的阿尔茨海默氏型老年痴呆症”Brain and Nerve 45. 455-460 (1993)。

Ikeda k et al.: "Neuropathological analysis of alcoholics with protracted delirium" Neurology Psychiatry and Brain Research. 1. 217-219 (1993)

Ikeda k 等人：“酗酒者长期谵妄的神经病理学分析”神经病学精神病学和大脑研究。