Surface modification for realizing implantable biosensors

用于实现植入式生物传感器的表面修饰

• 批准号: 02650294
• 负责人: IKEDA Kenji
• 金额: $ 1.41万
• 依托单位: University of Tokyo, Faculty of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02650294/
• 关键词: Ion-sensive field-effect transistro; Plasma-polymerized mambrane; SH-groups; Immobilized antibody; Non-specific adsorption; Surface potential; 有機膜; プラズマ重合; ヘキサメチルジシロキサン; 電界効果トランジスタ; 親水化; アミノ基

It is important for implantable immune-sensors by using ion-sensitive field-effect transistor(ISFET)to immobilize antibody-protein with high density and high activity. In this research, we use plasmapolymerized membrane for the career of inunobilized antibody. We use Hexamethyldisiloxane(HMDS), which is excellent in adhesion to silicon substrate, for the materials of organic membrane, and fabricate the membrane by plasma-polymerized method. We introduce SH-groups on the surface of organic membrane as functional group to inunobilize antibody by applying glow-discharge treatment to ethanedithiol having SH-groups. We confirm that hydrohibic surface becomes hydrophobic by this surface modification. The contact angle of the surface tension, which was 125ﾟ with water, turns 52ﾟ after modification. The fact that XPS signals of sulfur is observed only in the modificated membrane, is the evidence that SH-groups are introduced. Cysteine is covalently immobilized by SS-bond on the surface. The an … More tibody(anti-Human Serum Albrffin IgG)is covalently immobilized with crosslinking NH_2-groups of cysteine and SH-groups of antibody F_<ab>' hinge together with bifunctional reagent. The activity immobilized activated antibody density is of the order of 10^<11>[cm^<-2>](the closest density is 2X10^<12>)by enzymeimmunoassey(EIA), which shows high density immobilization. To investigate quantity of non-specific adsorption, organic membrane having no immobilized antibody is similarly measured by EIA. As a result, the membrane introduced SH-groups and Cysteine has less non-specific adsorption than that of NH_2-groups. We measure the changing of surface potential of this immobilized antibody caused by antigen-antibody binding. A surface potential change of the order of 0.1 mV is observed at the antigen concentration of 0.01 - 1 mg/ml. We attain the same result in the case that the antigen coexists with other substances(Bovine Serum). This result suggests the possibility of realizing immune-sensors. Less

利用离子敏感场效应晶体管(ISFET)高密度、高活性地固定化抗体蛋白对植入式免疫传感器具有重要意义。在这项研究中，我们使用胞浆多聚膜作为免疫抗体的职业。我们采用与硅衬底具有良好粘附性的六甲基二硅氧烷(HMDS)作为有机膜的材料，采用等离子体聚合的方法制备了有机膜。我们在有机膜表面引入SH基团作为官能团，对含有SH基团的乙二硫醇进行辉光放电处理，使抗体固定化。我们证实，通过这种表面修饰，疏水表面变得疏水。改性后的表面张力与水的接触角由原来的12 5ﾟ变为52ﾟ。硫的XPS信号仅在改性膜中观察到，这是SH基团引入的证据。半胱氨酸通过表面的SS-键共价固定。The An…更多的抗体(抗人血清蛋白Ig G)被半胱氨酸的NH_2-基团和抗体的SH-基团用双功能试剂铰接在一起。酶免疫法(EIA)的活性固定化抗体密度为10×10~(-1)~(-2)~(-2)数量级(最接近2×10~(-2)~(-2)~(-1))，具有较高的固定化密度。为了考察非特异性吸附的量，同样用EIA法测量了没有固定化抗体的有机膜。结果表明，引入SH基团和半胱氨酸的膜的非特异性吸附比引入NH_2-基的膜少。我们测量了抗原-抗体结合引起的该固定化抗体表面电位的变化。当抗原浓度为0.01-1 mg/ml时，表面电势变化约为0.1 mV，与其它物质(牛血清)共存时也有相同的结果。这一结果表明了实现免疫传感器的可能性。较少

项目成果

鈴木 誠一: "Surface Modification of ImmunoーSemiconductor Sensor" Proceeding of Far Eastern Conference on Medical and Biological Engineering 1990. 272-273 (1990)

Seiichi Suzuki：“免疫半导体传感器的表面修饰”远东医学和生物工程会议论文集 1990. 272-273 (1990)

六車 仁志: "プラズマ重合膜を用いた免疫センサ実現のための表面電位の測定" 第39回応用物理学関係連合講演会. (1992)

Hitoshi Muguruma：“使用等离子体聚合膜实现免疫传感器的表面电势测量”第 39 届应用物理协会会议（1992 年）。

H. Muguruma: "Measurement of Surface Potential for Immuno-sensors using Plasma-Polymerized mumbrane" Extended Abstracts (The 39th Spring Meeting, 1992) The Japan Soc, of Appl. Phys and Related Societies.

H. Muguruma：“使用等离子体聚合膜测量免疫传感器的表面电势”扩展摘要（第 39 届春季会议，1992 年）

鈴木 誠一: "有機膜表面改質による固定化抗体の活性保持" 医用電子と生体工学特別号 第29回日本ME学会論文集. 28. 84-84 (1990)

Seiichi Suzuki：“通过有机膜的表面修饰保留固定抗体的活性”医疗电子和生物工程特刊，第 29 届日本 ME 学会会议记录 28. 84-84 (1990)。