Identification and the elucidation of the mechanism of interleukin 18 induced metastasis inhibitory factor(s)

我们在之前的文章中表明了il -18诱导的转移抑制因子(s)存在的可能性。本研究的目的是鉴定该因子并阐明其抑制转移的机制。我们发现该因子主要存在于血清中。我们使用抗体阵列找到了32个候选因子。我们对5个高表达因子的转移抑制活性进行了研究，其中3个抑制了肿瘤细胞的侵袭。进一步，我们通过双向电泳和MALDI-TOF-MS发现了42个候选因子。

We showed the possibility of the presence of IL-18-induced metastasis inhibitory factor(s) in the previous paper. The purpose of this research is to identify the factor(s) and to clarify its metastasis suppression mechanism. We revealed that this factor mainly existed in serum. We found 32 candidates of the factor(s) using the antibody arrays. As a result of investigating the metastasis suppressing activity of five factors with high expression level, three of them inhibited the tumor cell invasion. Further, we found 42 candidates of the factor(s) through two-dimensional electrophoresis and the MALDI-TOF-MS.