Effect of radiofrequency hyperthermia on pain thresholds in osteoarthritis

射频热疗对骨关节炎痛阈的影响

• 批准号: 23590723
• 负责人: TAKAHASHI Kenji
• 金额: $ 3.24万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590723/
• 关键词: 変形性関節症; 温熱療法; 動物モデル; ラジオ波; 国際情報交換; 米国; 温熱慮法

The purpose of this study was to clarify the mechanism underlying the therapeutic effects of radiofrequency hyperthermia in naturally occurring OA of Hartley guinea pigs by investigating the level of autophagy. Thermotron-RF300, which can apply a 13.56 MHz radio frequency pulse to the animal knees was used to perform hyperthermia. The quantitative analysis of the distribution of positive cells showed that, in the normal cartilage, ULK1 and Beclin1 were highly expressed. Seven days after the exposure to the radiofrequency, the expression levels of ULK1 and Beclin1 were augmented in animals with moderate OA. The severity of cartilage degradation was significantly reduced in the radiofrequency-treated knees compared with the untreated left knees. These results suggest that radiofrequency hyperthermia can stimulate autophagy, which is reduced in OA cartilage. In addition, radiofrequency reduced the severity of spontaneous osteoarthritis.

本研究的目的是通过研究自噬水平来阐明射频热疗对Hartley豚鼠自然发生的OA的治疗作用的机制。Thermotron-RF 300可向动物膝关节施加13.56 MHz射频脉冲，用于进行热疗。对阳性细胞分布的定量分析表明，在正常软骨中，ULK 1和Beclin 1呈高表达。暴露于射频后7天，中度OA动物中ULK 1和Beclin 1的表达水平增加。与未治疗的左膝相比，射频治疗的膝关节软骨退化的严重程度显著降低。这些结果表明，射频热疗可以刺激自噬，这是减少OA软骨。此外，射频降低了自发性骨关节炎的严重程度。

项目成果

Efficacy of radiofrequency hyperthermia on the cartilage in guinea pigs with primary osteoarthritis

射频热疗对原发性骨关节炎豚鼠软骨的疗效

• 发表时间: 2013
• 作者: Kenji Takahashi;Hiroshi Watanabe;Yusuke Mochizuki;Sanshiro Hashimoto;Yoshihiko Satake;Hiroshi Nakamura;Shinro Takai
• 通讯作者: Shinro Takai

Efficacy of radiofrequency hyperthermia (poster)

射频热疗的功效（海报）

• 发表时间: 2011
• 作者: Takahashi K;Kurosaki H;Hashimoto S;et al.
• 通讯作者: et al.

関節軟骨老化の病態と電磁波温熱療法の効果

关节软骨老化的病理学及电磁波热疗的效果

• 发表时间: 2012
• 作者: 手塚 優子;加賀田 敬郎;加賀田 優紀;村尾 紀久子;楠目 和代;畑中 道代;北野 悦子;北村 肇;尾田 高志;高橋謙治，高井信朗，加藤和夫，新藤康弘，黒崎弘正，中村 洋
• 通讯作者: 高橋謙治，高井信朗，加藤和夫，新藤康弘，黒崎弘正，中村 洋

関節軟骨細胞の老化とストレス応答を利用した変形性関節症治療

利用关节软骨细胞的衰老和应激反应治疗骨关节炎

• 发表时间: 2011
• 期刊: 日本医大誌
• 作者: 内田 貴大;尾田 高志;武智 華子;渡辺 篤史;大島 直紀;堀田 修;島崎 秀幸;玉井 誠一;熊谷 裕生;曽我部昌一,川上順子;佐藤純;高橋謙治
• 通讯作者: 高橋謙治

Efficacy of radio frequency hyperthermia on the cartilage in guinea pigs with primary osteoarthritis

射频热疗对原发性骨关节炎豚鼠软骨的疗效

• 发表时间: 2013
• 作者: Kuwana S;Hosokawa Y;Sugita T;Umezawa N;Matsumoto A;Arisaka H;Yoshida K.;Kenji Takahashi
• 通讯作者: Kenji Takahashi