Global gene expression analysis of mechanical hyperalgesia induced by repeated cold stress in rats

已知反复冷应激（RCS）可短暂诱导与低血压和痛觉过敏相关的自主神经失衡。这种痛觉过敏被认为是由疼痛抑制系统功能低下引起的。本研究在SHRSP5/Dmcr大鼠中，采用基于pcr的cDNA减法分析和DNA芯片分析两种方法检测疼痛相关基因在背根神经节细胞（DRGs）中的表达变化。我们发现在rcs后，8个疼痛相关基因的表达显著增加或减少。DRG中至少有一些基因可能在RCS诱导的痛觉过敏中起关键作用。

Repeated cold stress (RCS) is known to transiently induce autonomic imbalance associated with hypotension and hyperalgesia. This hyperalgesia is thought to be caused by hypofunction of pain inhibitory systems. In this study, we detected changes in pain-related genes expression in the dorsal root ganglion cells (DRGs) by means of two methods, PCR-based cDNA subtraction analysis and DNA microarray analysis in SHRSP5/Dmcr rats. We found significant increase or decrease expressions in eight pain related genes at post-RCS. At least some of these genes in DRG may play key roles to hyperalgesia induced by RCS.