Development of an in vitro model system for drug metabolism studies with human hepatocyte

通过将肝细胞培养物中设计药物的代谢模式与体内研究的代谢模式进行比较，评估了肝细胞球体阵列试剂盒的实用性，该试剂盒使简单的三维肝细胞培养成为预测设计药物代谢命运的工具。在肝细胞球体的培养基中也检测到了所测试的设计药物的体内主要代谢物，表明使用肝细胞球体阵列试剂盒成功再现了设计药物的体内代谢模式。本研究开发的体外系统被发现是预测设计药物代谢命运的有效工具。

The usefulness of a hepatocyte spheroid array kit that enables simple three-dimensional hepatocyte culture as a tool to predict the metabolic fate of designer drugs was evaluated by comparing the metabolic pattern of designer drugs in hepatocyte culture to that of in vivo studies. The in vivo main metabolites of the designer drugs tested were also detected in the culture medium of the hepatocyte spheroids, indicating that the in vivo metabolic patterns of designer drugs were successfully reproduced using the hepatocyte spheroid array kit. The in vitro system developed in this study was found to be an effective tool for predicting the metabolic fate of designer drugs.