Development of an in vitro model system for drug metabolism studies with human hepatocyte

人肝细胞药物代谢研究体外模型系统的开发

• 批准号: 23590866
• 负责人: KANAMORI Tatsuyuki
• 金额: $ 3万
• 依托单位: National Research Institute of Police Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590866/
• 关键词: 乱用薬物; 代謝; 肝細胞; スフェロイド; 代謝予測; 三次元培養

The usefulness of a hepatocyte spheroid array kit that enables simple three-dimensional hepatocyte culture as a tool to predict the metabolic fate of designer drugs was evaluated by comparing the metabolic pattern of designer drugs in hepatocyte culture to that of in vivo studies. The in vivo main metabolites of the designer drugs tested were also detected in the culture medium of the hepatocyte spheroids, indicating that the in vivo metabolic patterns of designer drugs were successfully reproduced using the hepatocyte spheroid array kit. The in vitro system developed in this study was found to be an effective tool for predicting the metabolic fate of designer drugs.

通过比较设计药物在肝细胞培养和体内研究中的代谢模式，评估了肝细胞球状阵列试剂盒作为预测设计药物代谢命运的工具的有效性，该试剂盒能够实现简单的三维肝细胞培养。在肝细胞球体的培养液中也检测到设计药物的体内主要代谢产物，表明利用肝细胞球体阵列试剂盒成功地再现了设计药物的体内代谢模式。这项研究中开发的体外系统被发现是预测设计药物代谢命运的有效工具。

项目成果

Hepatocyte spheroid array kit as a tool for predicting in vivo drug metabolism

肝细胞球体阵列试剂盒作为预测体内药物代谢的工具

• 发表时间: 2014
• 作者: Kanamori;T. et al.
• 通讯作者: T. et al.

In vitro metabolism study of designer drugs using a hepatocyte spheroid array kit

使用肝细胞球体阵列试剂盒进行设计药物的体外代谢研究

• 发表时间: 2013
• 作者: Kanamori;T. et al.
• 通讯作者: T. et al.

肝細胞スフェロイドアレイ培養キットの新規乱用薬物代謝予測への応用

肝细胞球阵列培养试剂盒在新型滥用药物代谢预测中的应用

• 发表时间: 2013
• 作者: Saka K;Kudo K;Hayashida M;Kurisaki E;Niitsu H;Terada M;Yamaguchi K;Yoshida K;金森達之他
• 通讯作者: 金森達之他