Evaluation of 14C(11C)-Benzyl acetate as a potential probe for the measurement of glial metabolism.

为了评价[1- 14 C]乙酸苄酯（[1 - 14 C]BA）在活体测定胶质细胞氧化代谢中的有效性，采用解剖法研究了注射[1- 14 C] BA后小鼠和大鼠脑内的动力学。在示踪剂注射后，立即在小鼠和大鼠中观察到非常高的脑摄取。脑内[1- 14 C]BA放射性呈单指数曲线消失。[1- 14 C]BA的洗脱速率常数（k2）明显高于[2- 14 C]乙酸苄酯。k2值似乎是标记乙酸盐转化为标记CO2的转化率的指标。利用简化的放射自显影技术，可以估计出各脑区的k2值。以[1 - 11 C]乙酰氯为原料合成了[1 - 11 C]BA。用选择性胶质毒素预处理的麻醉或清醒大鼠进行了[1- 11 C]BA的PET测量，但在未来的研究中需要进行一些改进。

In order to evaluate the validity of benzyl [1-14C]acetate ([1-14C]BA) for in vivo measurement of glial oxidative metabolism, kinetics in the brain of mice or rats following injection of the tracer were studied by using the dissection method. Immediately after the tracer injection, a very high brain uptake was seen in mice and rats. Radioactivity of [1-14C]BA in the brain was disappeared as a single exponential curve. The washout rate constant (k2) of [1-14C]BA was considerably higher than that of benzyl [2-14C]acetate. The k2 value seems to be an index of conversion rate of labeled acetate to labeled CO2. By using simplified autoradiography, k2 value in each brain regions of interest could be estimated. [1-11C]BA was synthesized by using [1-11C]acetyl chloride. PET measurement of [1-11C]BA using anaesthetized or awake rats pretreated with selective glial toxin was performed, however several improvements are needed in future study.