Evaluation of 14C(11C)-Benzyl acetate as a potential probe for the measurement of glial metabolism.

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In order to evaluate the validity of benzyl [1-14C]acetate ([1-14C]BA) for in vivo measurement of glial oxidative metabolism, kinetics in the brain of mice or rats following injection of the tracer were studied by using the dissection method. Immediately after the tracer injection, a very high brain uptake was seen in mice and rats. Radioactivity of [1-14C]BA in the brain was disappeared as a single exponential curve. The washout rate constant (k2) of [1-14C]BA was considerably higher than that of benzyl [2-14C]acetate. The k2 value seems to be an index of conversion rate of labeled acetate to labeled CO2. By using simplified autoradiography, k2 value in each brain regions of interest could be estimated. [1-11C]BA was synthesized by using [1-11C]acetyl chloride. PET measurement of [1-11C]BA using anaesthetized or awake rats pretreated with selective glial toxin was performed, however several improvements are needed in future study.