Application of oncolytic adenovirus to the Exfoliative cytodiagnosis

溶瘤腺病毒在脱落细胞诊断中的应用

• 批准号: 22792015
• 负责人: INOUE Osamu
• 金额: $ 2.5万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22792015/
• 关键词: 口腔癌; ウイルス治療; 擦過細胞診; テロメラーゼ

In this study, the aim of clinical application of diagnosis and treatment of basic research that targets oral squamous cell carcinoma, using teromerase specific oncolytic adenovirus. A result, it is currently infected with Telomescan to human oral squamous cell carcinoma cell line, we studied possible visualization, GFP fluorescence was observed over a period of time coloring. Therefore, we search for the possibility of visualization of cancer cells in clinical specimens. The tumor as a model of oral cancer patients, in which oral squamous cell carcinoma cells were seeded in oral normal mucosa of healthy persons, were infected with Telomescan showed a color fluorescence of GFP.

本研究旨在对口腔鳞癌的临床应用诊断和治疗进行基础研究，即针对口腔鳞癌，采用端粒酶特异性溶瘤腺病毒。结果，它是目前用Telomescan感染到人口腔鳞癌细胞系中，我们研究了可能的可视化，观察到GFP荧光在一段时间内着色。因此，我们寻找在临床标本中的癌细胞的可视化的可能性。作为口腔癌患者模型的肿瘤，其中口腔鳞状细胞癌细胞接种在健康人的口腔正常粘膜中，用端粒扫描感染显示GFP的彩色荧光。