Proteomic analysis for RTK regulation by nitric oxide and its application to cancer therapy

一氧化氮RTK调节的蛋白质组学分析及其在癌症治疗中的应用

• 批准号: 23592016
• 负责人: SUGITA Hiroki
• 金额: $ 3.33万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592016/
• 关键词: RTK; 一酸化窒素(NO); IGF-iR; IRS-1; PI3-K/Akt; Yeast two hybrid assay; 膵癌; IGF-I; PI3-K/ Akt; NO; EGFR; MEK; MAPK; 増殖シグナル; 癌; PI3-K

To clarify the mechanisms of insulin-like growth factor(IGF) signaling regulated by nitric oxide, we performed Yeast Two Hybrid Assay which can search a protein binding to insulin receptor substrate( IRS)-1. Screening for cDNA library was performed. Eight proteins were selected as candidate of regulator of IRS-I. We found out that one of them bind to IRS-1 and regulated IGF signaling.

为了阐明一氧化氮对胰岛素样生长因子（insulin-like growth factor，IGF）信号转导的调控机制，我们采用酵母双杂交技术，寻找与胰岛素受体底物（insulin receptor substrate，IRS）-1结合的蛋白质。进行cDNA文库的筛选。筛选出8个蛋白质作为IRS-I的候选调控因子。我们发现其中一个与IRS-1结合并调节IGF信号传导。

项目成果

Statins inhibit tumor progression via an enhancer of zeste homolog 2-mediated epigenetic alteration in colorectal cancer.

• DOI: 10.1002/ijc.28672
• 发表时间: 2014-12-01
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Ishikawa, S.;Hayashi, H.;Kinoshita, K.;Abe, M.;Kuroki, H.;Tokunaga, R.;Tomiyasu, S.;Tanaka, H.;Sugita, H.;Arita, T.;Yagi, Y.;Watanabe, M.;Hirota, M.;Baba, H.
• 通讯作者: Baba, H.