Early diagnosis of prostate cancer using cancer-specific the glycosylated peptide antibody

使用癌症特异性糖基化肽抗体早期诊断前列腺癌

• 批准号: 23592350
• 负责人: FUJIMURA Tsutomu
• 金额: $ 3.16万
• 依托单位: Juntendo University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592350/
• 关键词: 悪性前立腺がん; ハプトグロビン; 糖鎖修飾ペプチド; ペプチド抗体; がん特異的抗体; シアル酸化; フコシル化; ELISA; シアル酸; ＥＬＩＳＡ法; PSA値; 国際情報交流

[Purpose] I found haptoglobin who received sugar chain modification cancer-specific from malignant prostate cancer patients serum. The antibody was produced to antigen glycosylated peptide from a cancer patient serum-derived haptoglobin. I aimed at the early diagnosis of prostate cancer. [Result] Antibody produced was possible to distinguish healthy control and malignant prostate cancer patients. To also distinguish between malignant prostate cancer and benign disease patients was also possible. The prototype that can be measured by ELISA was able to sufficient sensitivity is obtained.It was detectable the number ng / ml sugar chain haptoglobin in serum. I was able to produce ELISA to make up for the shortcomings of PSA levels. Furthermore, we want to raise the accuracy of the early diagnosis method by increase the number of samples.

[目的] 我从恶性前列腺癌患者的血清中发现了经过糖链修饰的癌症特异性触珠蛋白。该抗体是针对来自癌症患者血清的触珠蛋白的抗原糖基化肽而产生的。我的目标是前列腺癌的早期诊断。 [结果]产生的抗体可以区分健康对照和恶性前列腺癌患者。区分恶性前列腺癌和良性疾病患者也是可能的。 获得了能够通过ELISA测量的原型，能够具有足够的灵敏度。可检测血清中的糖链触珠蛋白的数量ng/ml。我能够制作ELISA来弥补PSA水平的缺陷。此外，我们希望通过增加样本数量来提高早期诊断方法的准确性。

项目成果

Oligosaccharide structure analysis of Acute Phase Protein in the serum of the prostate cancer patient

前列腺癌患者血清中急性相蛋白的寡糖结构分析

• 发表时间: 2013

Metabolic shift in hypoxia-adapted CML cells and mechanisms of acquired dasatinib resistance uncovered by proteomic iTRAQ profiling

蛋白质组 iTRAQ 分析揭示缺氧适应 CML 细胞的代谢变化和获得性达沙替尼耐药机制

• 发表时间: 2013

Liver autophagy contributes to the maintenance of blood glucose and amino acid levels

• DOI: 10.4161/auto.7.7.15371
• 发表时间: 2011-07-01
• 期刊: AUTOPHAGY
• 影响因子: 13.3
• 作者: Ezaki, Junji;Matsumoto, Naomi;Ueno, Takashi
• 通讯作者: Ueno, Takashi

Galectin-4, a novel predictor for lymph node metastasis in lung adenocarcinoma.

• DOI: 10.1371/journal.pone.0081883
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Hayashi T;Saito T;Fujimura T;Hara K;Takamochi K;Mitani K;Mineki R;Kazuno S;Oh S;Ueno T;Suzuki K;Yao T
• 通讯作者: Yao T

Integrative genomic and proteomic analysis of low-dose ionizing irradiation effects on bone marrow stromal microenvironment and on survival of pre-leukemic cells.

低剂量电离辐射对骨髓基质微环境和白血病前细胞存活影响的综合基因组和蛋白质组分析。

• 发表时间: 2013
• 作者: Tabe Y;Jin L;Hatanaka Y;Matsushita H;Kazuno S;Fujimura T;Ueno U;Sasai K;Iwabuchi K;Andreeff M;Konopleva M;MD;Miida T.
• 通讯作者: Miida T.