Expression and its functional role of Beta3-adrenoceptors in the human prostate

人前列腺中β3-肾上腺素受体的表达及其功能作用

• 批准号: 23592365
• 负责人: OTSUKA Atsushi
• 金额: $ 3.49万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592365/
• 关键词: 前立腺; β-アドレナリン受容体; ヒト; β3-アドレナリン受容体; 平滑筋; β‐アドレナリン受容体; β3‐アドレナリン受容体

Expression of beta1-, beta2- and beta3-AR mRNA in the human prostate was confirmed by RT-PCR. On immunohistochemistry, positive staining for beta1-, beta2- and beta3-AR was identified not only in prostatic smooth muscles, but also glandular epithelium. In pharmacological study in vitro, a beta3-AR agonist concentration-dependently decreased KCL- or EFS-induced contraction of the human normal prostatic strips. In conclusion, beta3-AR agonists may have clinical benefit for lower urinary tract symptom due to either benign prostatic hyperplasia or neurogenic bladder.

RT-PCR证实了β 1-、β 2-和β 3- ar mRNA在人前列腺中的表达。免疫组化结果显示，β a1-、β a2-和β a3- ar不仅在前列腺平滑肌中呈阳性，而且在腺上皮中也呈阳性。在体外药理学研究中，β 3- ar激动剂浓度依赖性地降低了KCL-或efs诱导的人正常前列腺条的收缩。综上所述，β 3- ar激动剂可能对良性前列腺增生或神经源性膀胱引起的下尿路症状有临床益处。