Age-Related Changes in the Stria Vascularis in C57BL/6 mouse

C57BL/6 小鼠血管纹的年龄相关变化

• 批准号: 23592500
• 负责人: SUZUKI Mitsuya
• 金额: $ 3.33万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592500/
• 关键词: C57BL/6 マウス; 加齢性難聴; polyethyleneimine; 陰性荷電; IgG; 免疫組織学; 血管条; 毛細血管基底膜; 血液―内耳関門; 毛細血管; 基底膜; ヘパラン硫酸プロテオグリカン; 基底膜陰性荷電; C57BL/6マウス; 蝸牛; ヘパラン硫酸

Purpose: To examine age-related morphological changes in the cochlear lateral wall. Methods: C57BL/6 mice were grouped according to age as follows: 3 days, 4 weeks, 8 weeks, 6months and 12 months. Polyethyleneimine (PEI) was used to examine changes in the basement membrane anionic sites(BMAS) of the strial vessels. Serial mid-modiolarparaffin thin sections were performed to measure the widths of the stria vascularis, and then incubate with anti-mouse IgG antibody. Results: In a width of the stria vascularis, a significant difference was observed between the 4- week- and 12- month-old mice. A statistically significant difference was observed in the distribution of PEI particles between 3-day- and 8-week-, 6- and 12- month-old mice. IgG deposition was detected in the strial capillary BM in 8-week-old, 6- month-old, and 12-month-old mice. Since IgG is a cationized antibody molecule, the decrease in the BMAS shown may be due to IgG deposition in the strial vessels.

目的：探讨耳蜗外侧壁的年龄相关性形态学改变。方法：C57BL/6小鼠按年龄分组：3日龄、4周龄、8周龄、6月龄、12月龄。用聚乙烯亚胺（PEI）检测大鼠基底膜阴离子位点（BMAS）的变化。连续中蜡切片测量血管纹宽度，并用抗小鼠IgG抗体孵育。结果：在血管纹宽度上，4周龄和12月龄小鼠有显著差异。在3天和8周、6个月和12个月大的小鼠中，PEI颗粒的分布有统计学上的显著差异。8周龄、6月龄和12月龄小鼠毛细血管BM中均检测到IgG沉积。由于IgG是一种阳离子化的抗体分子，BMAS的下降可能是由于IgG在大鼠血管中的沉积。

项目成果

Age-Related Morphological Changes in the Stria Vascularis in C57BL/6.

C57BL/6 中血管纹与年龄相关的形态变化。

• 发表时间: 2014
• 作者: Mitsuya Suzuki;Takashi Sakamoto;Yohihiro Ikemiyagi;Akinori Kashio;Tatsuya Yamasoba :
• 通讯作者: Tatsuya Yamasoba :

蝸牛の基底膜陰性荷電に対する加齢の影響

衰老对耳蜗基底膜负电荷的影响

• 发表时间: 2012
• 作者: 鈴木光也;坂本幸士;樫尾明憲;池宮城慶寛;山岨達也
• 通讯作者: 山岨達也

Age-Related Changes in Basement Membrane Anionic Sites in the Cochlea

耳蜗基底膜阴离子位点与年龄相关的变化

• 发表时间: 2012
• 作者: Mitsuya Suzuki;Takashi Sakamoto;Akinori Kashio;Yoshihiro Ikemiyagi;Tatsuya Yamasoba
• 通讯作者: Tatsuya Yamasoba

蝸牛の基底膜陰性荷電に対する 加齢の影響

衰老对耳蜗基底膜负电荷的影响

• 作者: Mitsuya Suzuki;Takashi Sakamoto; Yoshihiro Ikemiyagi;Akinori Kashio; Tatsuya Yamasoba;鈴木光也,坂本幸士,樫尾明憲,池宮城慶寛,山岨達也
• 通讯作者: 鈴木光也,坂本幸士,樫尾明憲,池宮城慶寛,山岨達也

Short-term functional and morphological changes in guinea pig cochlea following intratympanic application of Burow’s solution

鼓室内应用 Burow 溶液后豚鼠耳蜗的短期功能和形态变化

• 发表时间: 2012
• 期刊: Ann Otol. Rhinol. Laryngol
• 作者: Suzuki M;Iwamura H;Kashio A;Sakamoto T;Yamasoba T.
• 通讯作者: Yamasoba T.